3D image reconstruction of transparent microscopic objects using digital holography

Francisco Palacios, Jorge Ricardo, Daniel Palacios, Edison Gonçalves, Jose L. Valin, Rodrigo De Souza

Research output: Contribution to journalArticlepeer-review

62 Scopus citations


In this paper, we present the potentialities of the digital holography microscopy for 3D image reconstruction of transparent microscopic objects. A method for object volume reconstruction based on the capture of only one off-axis hologram is discussed. We show that this technique can be efficiently used for obtaining quantitative information from the intensity and the phase distributions of the reconstructed field at different locations along the propagation direction. The potential of the method has been exploited by applying the 3D image reconstruction procedure to etched nuclear tracks induced in the widely used polymer detector CR-39. Nowadays, confocal microscopy and atomic force microscopy are applied to study nuclear track morphology in CR-39 detectors. The micro-holographic method developed in this work, and applied to that particular study, constitutes a new alternative procedure that overcomes the previously described at least in technological simplicity.

Original languageEnglish
Pages (from-to)41-50
Number of pages10
JournalOptics Communications
Issue number1-3
StatePublished - 1 Apr 2005
Externally publishedYes


  • 3D phase reconstruction
  • Digital holography
  • Holographic contouring
  • Phase unwrapping


Dive into the research topics of '3D image reconstruction of transparent microscopic objects using digital holography'. Together they form a unique fingerprint.

Cite this