A combined protein toxin screening based on the transcriptome and proteome of Solenopsis invicta

Liuyang Cai, Fengling Yang, Yongfang Wang, Jishun Yang, Yina Zhu, Xueqi Ma, Juan Höfer, Yichao Wang, Yajun Ma, Liang Xiao

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Multi-omics technology provides a good tool to analyze the protein toxin composition and search for the potential pathogenic factors of Solenopsis invicta, under the great harm of the accelerated invasion in southern China. Methods: Species collection, functional annotation, toxin screening, and 3D modeling construction of three interested toxins were performed based on the successfully constructed transcriptome and proteome of S. invicta. Results: A total of 33,231 unigenes and 721 proteins were obtained from the constructed transcriptome and proteome, of which 9,842 (29.62%) and 4,844 (14.58%) unigenes, as well as 469 (65.05%) and 71 (99.45%) proteins were annotated against the databases of Gene Ontology and Kyoto Encyclopedia of Genes and Genomes, respectively. After comparing with the uniprot toxin database, a total of 316 unigenes and 47 proteins (calglandulin, venom allergen 3, and venom prothrombin activator hopsarin-D, etc.) were successfully screened. Conclusions: The update of annotations at the transcriptome and proteome levels presents a progression in the comprehension of S. invicta in China. We also provide a protein toxin list that could be used for further exploration of toxicity as well as its antagonistic strategy by S. invicta.

Original languageEnglish
Article number15
JournalProteome Science
Volume20
Issue number1
DOIs
StatePublished - Dec 2022
Externally publishedYes

Keywords

  • Ant
  • Proteome
  • Solenopsis invicta
  • Toxin
  • Transcriptome

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