A natural peptide and its variants derived from the processing of infectious pancreatic necrosis virus (IPNV) displaying enhanced antimicrobial activity: A novel alternative for the control of bacterial diseases

Claudio Jofré; Fanny Guzmán; Constanza Cárdenas; Fernando Albericio; Sergio H. Marshall

doi:10.1016/j.peptides.2011.01.026

A natural peptide and its variants derived from the processing of infectious pancreatic necrosis virus (IPNV) displaying enhanced antimicrobial activity: A novel alternative for the control of bacterial diseases

Claudio Jofré, Fanny Guzmán, Constanza Cárdenas, Fernando Albericio, Sergio H. Marshall

INSTITUTE OF BIOLOGY

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

The larger segment of the infectious pancreatic necrosis virus (IPNV) codifies most of the structural and non-structural proteins of the virus in two overlapping open reading frames (ORFs). The longer of the two ORF is expressed as a polyprotein which generates a number of variable length peptides of unknown function during processing. Since an appealing hypothesis would be that these peptides are generated by the virus to act as antimicrobial agents that favor viral infectivity in their fish host, we decided to test this possibility by selecting a master peptide and using it to generate substitution variants that may enhance their antimicrobial potential. A 20-residue master peptide (p20) was selected from the well-described maturation process of the structural viral protein VP2; several variants were then designed and chemically synthesized, ranging in size from 16 to 20 residues. The synthesized peptides were tested for in vitro activity against several prototype bacterial pathogens using standardized laboratory procedures. Chemically synthesized p20 and all its variants displayed broad activity against the tested bacteria and none of them were toxic to eukaryotic cells at least 10× the concentration used against the bacteria. Interestingly, when p20 was tested against the very aggressive bacterial pathogen Piscirickettsia salmonis, a common co-infectant of IPNV in salmonid fish, the specific activity of the novel peptide was significantly higher than that displayed for bactericidal fish farm antibiotics such as oxolinic acid, flumequine and florfenicol, which are commonly used to control Piscirickettsiosis in the field. It is potentially significant that the approach presented in this report provides a novel alternative for generating new and ideally more efficient and friendly safeguards for bacterial prophylaxis.

Original language	English
Pages (from-to)	852-858
Number of pages	7
Journal	Peptides
Volume	32
Issue number	5
DOIs	https://doi.org/10.1016/j.peptides.2011.01.026
State	Published - May 2011

Keywords

Antibacterial peptide
IPNV processing
Peptide modeling
Peptide synthesis
Viral derived antibacterial peptide

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title = "A natural peptide and its variants derived from the processing of infectious pancreatic necrosis virus (IPNV) displaying enhanced antimicrobial activity: A novel alternative for the control of bacterial diseases",

abstract = "The larger segment of the infectious pancreatic necrosis virus (IPNV) codifies most of the structural and non-structural proteins of the virus in two overlapping open reading frames (ORFs). The longer of the two ORF is expressed as a polyprotein which generates a number of variable length peptides of unknown function during processing. Since an appealing hypothesis would be that these peptides are generated by the virus to act as antimicrobial agents that favor viral infectivity in their fish host, we decided to test this possibility by selecting a master peptide and using it to generate substitution variants that may enhance their antimicrobial potential. A 20-residue master peptide (p20) was selected from the well-described maturation process of the structural viral protein VP2; several variants were then designed and chemically synthesized, ranging in size from 16 to 20 residues. The synthesized peptides were tested for in vitro activity against several prototype bacterial pathogens using standardized laboratory procedures. Chemically synthesized p20 and all its variants displayed broad activity against the tested bacteria and none of them were toxic to eukaryotic cells at least 10× the concentration used against the bacteria. Interestingly, when p20 was tested against the very aggressive bacterial pathogen Piscirickettsia salmonis, a common co-infectant of IPNV in salmonid fish, the specific activity of the novel peptide was significantly higher than that displayed for bactericidal fish farm antibiotics such as oxolinic acid, flumequine and florfenicol, which are commonly used to control Piscirickettsiosis in the field. It is potentially significant that the approach presented in this report provides a novel alternative for generating new and ideally more efficient and friendly safeguards for bacterial prophylaxis.",

keywords = "Antibacterial peptide, IPNV processing, Peptide modeling, Peptide synthesis, Viral derived antibacterial peptide",

author = "Claudio Jofr{\'e} and Fanny Guzm{\'a}n and Constanza C{\'a}rdenas and Fernando Albericio and Marshall, {Sergio H.}",

note = "Funding Information: This work was funded by the Government Grant CORFO-Innova Chile PTI-11 , AECID Spain Grant A/023783/09 , and CICYT ( CTQ2009-07758 ).",

year = "2011",

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doi = "10.1016/j.peptides.2011.01.026",

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Jofré, C, Guzmán, F, Cárdenas, C, Albericio, F & Marshall, SH 2011, 'A natural peptide and its variants derived from the processing of infectious pancreatic necrosis virus (IPNV) displaying enhanced antimicrobial activity: A novel alternative for the control of bacterial diseases', Peptides, vol. 32, no. 5, pp. 852-858. https://doi.org/10.1016/j.peptides.2011.01.026

A natural peptide and its variants derived from the processing of infectious pancreatic necrosis virus (IPNV) displaying enhanced antimicrobial activity: A novel alternative for the control of bacterial diseases. / Jofré, Claudio; Guzmán, Fanny; Cárdenas, Constanza et al.
In: Peptides, Vol. 32, No. 5, 05.2011, p. 852-858.

Research output: Contribution to journal › Article › peer-review

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T2 - A novel alternative for the control of bacterial diseases

AU - Jofré, Claudio

AU - Guzmán, Fanny

AU - Cárdenas, Constanza

AU - Albericio, Fernando

AU - Marshall, Sergio H.

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A natural peptide and its variants derived from the processing of infectious pancreatic necrosis virus (IPNV) displaying enhanced antimicrobial activity: A novel alternative for the control of bacterial diseases

Abstract

Keywords

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