A segmentation clock with two-segment periodicity in insects

Andres F. Sarrazin, Andrew D. Peel, Michalis Averof

Research output: Contribution to journalArticlepeer-review

155 Scopus citations


Vertebrate segmentation relies on a mechanism characterized by oscillating gene expression. Whether this mechanism is used by other segmented animals has been controversial. Rigorous proof of cyclic expression during arthropod segmentation has been lacking. We find that the segmentation gene odd-skipped (Tc-odd) oscillates with a two-segment periodicity in the beetle Tribolium castaneum. By bisecting embryos and culturing the two halves over different time intervals, we demonstrate that Tc-odd cycles with a period of about 95 minutes at 30°C. Using live imaging and cell tracking in green fluorescent protein-expressing embryos, we can exclude that cell movements explain this dynamic expression. Our results show that molecular oscillators represent a common feature of segmentation in divergent animals and help reconcile the contrasting paradigms of insect and vertebrate segmentation.

Original languageEnglish
Pages (from-to)338-341
Number of pages4
Issue number6079
StatePublished - 20 Apr 2012


Dive into the research topics of 'A segmentation clock with two-segment periodicity in insects'. Together they form a unique fingerprint.

Cite this