TY - JOUR
T1 - Advances in Cell Engineering of the Komagataella phaffii Platform for Recombinant Protein Production
AU - Bustos, Cristina
AU - Quezada, Johan
AU - Veas, Rhonda
AU - Altamirano, Claudia
AU - Braun-Galleani, Stephanie
AU - Fickers, Patrick
AU - Berrios, Julio
N1 - Publisher Copyright:
© 2022 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2022/4
Y1 - 2022/4
N2 - Komagataella phaffii (formerly known as Pichia pastoris) has become an increasingly important microorganism for recombinant protein production. This yeast species has gained high interest in an industrial setting for the production of a wide range of proteins, including enzymes and biopharma-ceuticals. During the last decades, relevant bioprocess progress has been achieved in order to increase recombinant protein productivity and to reduce production costs. More recently, the improvement of cell features and performance has also been considered for this aim, and promising strategies with a direct and substantial impact on protein productivity have been reported. In this review, cell engineering approaches including metabolic engineering and energy supply, transcription factor modulation, and manipulation of routes involved in folding and secretion of recombinant protein are discussed. A lack of studies performed at the higher-scale bioreactor involving optimisation of cultivation parameters is also evidenced, which highlights new research aims to be considered.
AB - Komagataella phaffii (formerly known as Pichia pastoris) has become an increasingly important microorganism for recombinant protein production. This yeast species has gained high interest in an industrial setting for the production of a wide range of proteins, including enzymes and biopharma-ceuticals. During the last decades, relevant bioprocess progress has been achieved in order to increase recombinant protein productivity and to reduce production costs. More recently, the improvement of cell features and performance has also been considered for this aim, and promising strategies with a direct and substantial impact on protein productivity have been reported. In this review, cell engineering approaches including metabolic engineering and energy supply, transcription factor modulation, and manipulation of routes involved in folding and secretion of recombinant protein are discussed. A lack of studies performed at the higher-scale bioreactor involving optimisation of cultivation parameters is also evidenced, which highlights new research aims to be considered.
KW - Komagataella phaffii
KW - Pichia pastoris
KW - cell engineering
KW - metabolic engineering
KW - recombinant protein
UR - http://www.scopus.com/inward/record.url?scp=85129118323&partnerID=8YFLogxK
U2 - 10.3390/metabo12040346
DO - 10.3390/metabo12040346
M3 - Review article
AN - SCOPUS:85129118323
SN - 2218-1989
VL - 12
JO - Metabolites
JF - Metabolites
IS - 4
M1 - 346
ER -