Advances in Cell Engineering of the Komagataella phaffii Platform for Recombinant Protein Production

Cristina Bustos, Johan Quezada, Rhonda Veas, Claudia Altamirano, Stephanie Braun-Galleani, Patrick Fickers, Julio Berrios

Research output: Contribution to journalReview articlepeer-review

1 Scopus citations

Abstract

Komagataella phaffii (formerly known as Pichia pastoris) has become an increasingly important microorganism for recombinant protein production. This yeast species has gained high interest in an industrial setting for the production of a wide range of proteins, including enzymes and biopharma-ceuticals. During the last decades, relevant bioprocess progress has been achieved in order to increase recombinant protein productivity and to reduce production costs. More recently, the improvement of cell features and performance has also been considered for this aim, and promising strategies with a direct and substantial impact on protein productivity have been reported. In this review, cell engineering approaches including metabolic engineering and energy supply, transcription factor modulation, and manipulation of routes involved in folding and secretion of recombinant protein are discussed. A lack of studies performed at the higher-scale bioreactor involving optimisation of cultivation parameters is also evidenced, which highlights new research aims to be considered.

Original languageEnglish
Article number346
JournalMetabolites
Volume12
Issue number4
DOIs
StatePublished - Apr 2022
Externally publishedYes

Keywords

  • cell engineering
  • Komagataella phaffii
  • metabolic engineering
  • Pichia pastoris
  • recombinant protein

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