TY - JOUR
T1 - Advances in Cell Engineering of the Komagataella phaffii Platform for Recombinant Protein Production
AU - Bustos, Cristina
AU - Quezada, Johan
AU - Veas, Rhonda
AU - Altamirano, Claudia
AU - Braun-Galleani, Stephanie
AU - Fickers, Patrick
AU - Berrios, Julio
N1 - Funding Information:
Funding: This research was funded by FONDECYT Regular grant number 1191196, FONDECYT Ini-ciación grant number 11200933, ANILLO Regular de Ciencia y Tecnología grant number ACT210068, and Becas Doctorado Nacional grant numbers 21191422, 21211138, 21211233—Agencia Nacional de Investigación y Desarrollo (ANID), Chile; and Wallonie-Bruxelles International through the Cooperation bilateral Belgique-Chili project SUB/2019/435787.
Funding Information:
This research was funded by FONDECYT Regular grant number 1191196, FONDECYT Iniciaci?n grant number 11200933, ANILLO Regular de Ciencia y Tecnolog?a grant number ACT210068, and Becas Doctorado Nacional grant numbers 21191422, 21211138, 21211233?Agencia Nacional de Investigaci?n y Desarrollo (ANID), Chile; and Wallonie-Bruxelles International through the Cooperation bilateral Belgique-Chili project SUB/2019/435787.
Publisher Copyright:
© 2022 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2022/4
Y1 - 2022/4
N2 - Komagataella phaffii (formerly known as Pichia pastoris) has become an increasingly important microorganism for recombinant protein production. This yeast species has gained high interest in an industrial setting for the production of a wide range of proteins, including enzymes and biopharma-ceuticals. During the last decades, relevant bioprocess progress has been achieved in order to increase recombinant protein productivity and to reduce production costs. More recently, the improvement of cell features and performance has also been considered for this aim, and promising strategies with a direct and substantial impact on protein productivity have been reported. In this review, cell engineering approaches including metabolic engineering and energy supply, transcription factor modulation, and manipulation of routes involved in folding and secretion of recombinant protein are discussed. A lack of studies performed at the higher-scale bioreactor involving optimisation of cultivation parameters is also evidenced, which highlights new research aims to be considered.
AB - Komagataella phaffii (formerly known as Pichia pastoris) has become an increasingly important microorganism for recombinant protein production. This yeast species has gained high interest in an industrial setting for the production of a wide range of proteins, including enzymes and biopharma-ceuticals. During the last decades, relevant bioprocess progress has been achieved in order to increase recombinant protein productivity and to reduce production costs. More recently, the improvement of cell features and performance has also been considered for this aim, and promising strategies with a direct and substantial impact on protein productivity have been reported. In this review, cell engineering approaches including metabolic engineering and energy supply, transcription factor modulation, and manipulation of routes involved in folding and secretion of recombinant protein are discussed. A lack of studies performed at the higher-scale bioreactor involving optimisation of cultivation parameters is also evidenced, which highlights new research aims to be considered.
KW - cell engineering
KW - Komagataella phaffii
KW - metabolic engineering
KW - Pichia pastoris
KW - recombinant protein
UR - http://www.scopus.com/inward/record.url?scp=85129118323&partnerID=8YFLogxK
U2 - 10.3390/metabo12040346
DO - 10.3390/metabo12040346
M3 - Review article
AN - SCOPUS:85129118323
VL - 12
JO - Metabolites
JF - Metabolites
SN - 2218-1989
IS - 4
M1 - 346
ER -