Alginate molecular mass produced by Azotobacter vinelandii in response to changes of the O2 transfer rate in chemostat cultures

ALVARO ENRIQUE DIAZ BARRERA, P. Silva, R. Ávalos, F. Acevedo

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19 Scopus citations

Abstract

Alginate production by Azotobacter vinelandii growing in chemostat cultures was evaluated under different O2 transfer rates (OTR). As a result of modifying the culture's agitation rate from 300 to 500 rpm, the OTR increased from 9 to 15.1 mmol l-1 h-1 and a slight variation in the alginate production (1.7-2.2 g l-1) was observed. At a constant growth rate (0.1 h-1), the mean molecular mass of the alginate was strongly influenced by changes in the OTR, varying from 860 to 1,690 kDa. These results support a possible relationship between alginate polymerization- depolymerization process and the O2 uptake rate.

Original languageEnglish
Pages (from-to)825-829
Number of pages5
JournalBiotechnology Letters
Volume31
Issue number6
DOIs
StatePublished - 1 Jun 2009

Keywords

  • Alginate
  • Chemostat
  • Molecular mass
  • Oxygen transfer rate

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