Amiloride fluxes across erythrocyte membranes

Dale J. Benos, JUAN GUILLERMO REYES MARTINEZ, David G. Shoemaker

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Amiloride is known to inhibit both the influx of Na+ and the activation of mitogensis in many cultured cell lines. This paper describes experiments in which the permeability coefficient of amiloride was determined from measurements of tracer fluxes across human erythrocytes and resealed ghosts. From an analysis of these fluxes, a permeability coefficient of 10-7 cm/s for the uncharged form of amiloride was deduced. Based upon this measured permeability value, we present calculations of intracellular accumulation times of amiloride in cells of differing surface-to-volume ratio.

Original languageEnglish
Pages (from-to)99-104
Number of pages6
JournalBiochimica et Biophysica Acta - Biomembranes
Volume734
Issue number1
DOIs
StatePublished - 21 Sep 1983
Externally publishedYes

Keywords

  • (Human blood)
  • Amiloride flux
  • Erythrocyte membrane
  • Na transport

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