Amiloride fluxes across erythrocyte membranes

Dale J. Benos; Juan Reyes; David G. Shoemaker

doi:10.1016/0005-2736(83)90080-9

Amiloride fluxes across erythrocyte membranes

Dale J. Benos, Juan Reyes, David G. Shoemaker

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

Amiloride is known to inhibit both the influx of Na⁺ and the activation of mitogensis in many cultured cell lines. This paper describes experiments in which the permeability coefficient of amiloride was determined from measurements of tracer fluxes across human erythrocytes and resealed ghosts. From an analysis of these fluxes, a permeability coefficient of 10^-7 cm/s for the uncharged form of amiloride was deduced. Based upon this measured permeability value, we present calculations of intracellular accumulation times of amiloride in cells of differing surface-to-volume ratio.

Original language	English
Pages (from-to)	99-104
Number of pages	6
Journal	Biochimica et Biophysica Acta - Biomembranes
Volume	734
Issue number	1
DOIs	https://doi.org/10.1016/0005-2736(83)90080-9
State	Published - 21 Sep 1983
Externally published	Yes

Keywords

(Human blood)
Amiloride flux
Erythrocyte membrane
Na transport

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10.1016/0005-2736(83)90080-9

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title = "Amiloride fluxes across erythrocyte membranes",

abstract = "Amiloride is known to inhibit both the influx of Na+ and the activation of mitogensis in many cultured cell lines. This paper describes experiments in which the permeability coefficient of amiloride was determined from measurements of tracer fluxes across human erythrocytes and resealed ghosts. From an analysis of these fluxes, a permeability coefficient of 10-7 cm/s for the uncharged form of amiloride was deduced. Based upon this measured permeability value, we present calculations of intracellular accumulation times of amiloride in cells of differing surface-to-volume ratio.",

keywords = "(Human blood), Amiloride flux, Erythrocyte membrane, Na transport",

author = "Benos, {Dale J.} and Juan Reyes and Shoemaker, {David G.}",

note = "Funding Information: bergh for typing this manuscript. This work was supported by NIH Grant No. AM-25886 and by funds from the Andrew W. Mellon Foundation.",

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AU - Benos, Dale J.

AU - Reyes, Juan

AU - Shoemaker, David G.

N1 - Funding Information: bergh for typing this manuscript. This work was supported by NIH Grant No. AM-25886 and by funds from the Andrew W. Mellon Foundation.

PY - 1983/9/21

Y1 - 1983/9/21

N2 - Amiloride is known to inhibit both the influx of Na+ and the activation of mitogensis in many cultured cell lines. This paper describes experiments in which the permeability coefficient of amiloride was determined from measurements of tracer fluxes across human erythrocytes and resealed ghosts. From an analysis of these fluxes, a permeability coefficient of 10-7 cm/s for the uncharged form of amiloride was deduced. Based upon this measured permeability value, we present calculations of intracellular accumulation times of amiloride in cells of differing surface-to-volume ratio.

AB - Amiloride is known to inhibit both the influx of Na+ and the activation of mitogensis in many cultured cell lines. This paper describes experiments in which the permeability coefficient of amiloride was determined from measurements of tracer fluxes across human erythrocytes and resealed ghosts. From an analysis of these fluxes, a permeability coefficient of 10-7 cm/s for the uncharged form of amiloride was deduced. Based upon this measured permeability value, we present calculations of intracellular accumulation times of amiloride in cells of differing surface-to-volume ratio.

KW - (Human blood)

KW - Amiloride flux

KW - Erythrocyte membrane

KW - Na transport

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DO - 10.1016/0005-2736(83)90080-9

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JO - Biochimica et Biophysica Acta - Biomembranes

JF - Biochimica et Biophysica Acta - Biomembranes

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ER -

Amiloride fluxes across erythrocyte membranes

Abstract

Keywords

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