An alternative method for a fast separation of phosphotyrosine

G. Muñoz, S. H. Marshall

Research output: Contribution to journalArticlepeer-review

48 Scopus citations


A simple and rapid procedure is described for fully separating phosphotyrosine from phosphoserine and phosphothreonine through one-dimensional thin-layer chromatography. The migration properties of these phosphoamino acids are compared with those of CMP, UMP, ATP, ribose phosphate, and inorganic orthophosphate, considered the most frequent comigrating products derived from 32P-labeling experiments. We demonstrate that Rf values for the three phosphoamino acids differ from those displayed by the mentioned contaminating compounds. One of the most relevant advantages of this procedure is that a complete separation of phosphotyrosine can be achieved in only 90 min.

Original languageEnglish
Pages (from-to)233-237
Number of pages5
JournalAnalytical Biochemistry
Issue number2
StatePublished - 1 Nov 1990


Dive into the research topics of 'An alternative method for a fast separation of phosphotyrosine'. Together they form a unique fingerprint.

Cite this