TY - JOUR
T1 - An alternative method for a fast separation of phosphotyrosine
AU - Muñoz, G.
AU - Marshall, S. H.
N1 - Funding Information:
We thank Dr. Ema Navarrete and Ms. Christa Seebach for providing PHA-stimulated human blood lymphocytes, Mr. C. Gonzalez for the art work, and Mr. G. Sierralta for assistance with the editing process. We are deeply indebted to Dr. Tony Hunter from the Salk Institute (La Jolla, CA) for his various suggestions and critical review of the manuscript. This research was supported by Direction General de Investigation, Universidad Catolica de Valparaiso, Chile.
PY - 1990/11/1
Y1 - 1990/11/1
N2 - A simple and rapid procedure is described for fully separating phosphotyrosine from phosphoserine and phosphothreonine through one-dimensional thin-layer chromatography. The migration properties of these phosphoamino acids are compared with those of CMP, UMP, ATP, ribose phosphate, and inorganic orthophosphate, considered the most frequent comigrating products derived from 32P-labeling experiments. We demonstrate that Rf values for the three phosphoamino acids differ from those displayed by the mentioned contaminating compounds. One of the most relevant advantages of this procedure is that a complete separation of phosphotyrosine can be achieved in only 90 min.
AB - A simple and rapid procedure is described for fully separating phosphotyrosine from phosphoserine and phosphothreonine through one-dimensional thin-layer chromatography. The migration properties of these phosphoamino acids are compared with those of CMP, UMP, ATP, ribose phosphate, and inorganic orthophosphate, considered the most frequent comigrating products derived from 32P-labeling experiments. We demonstrate that Rf values for the three phosphoamino acids differ from those displayed by the mentioned contaminating compounds. One of the most relevant advantages of this procedure is that a complete separation of phosphotyrosine can be achieved in only 90 min.
UR - http://www.scopus.com/inward/record.url?scp=0025133304&partnerID=8YFLogxK
U2 - 10.1016/0003-2697(90)90185-C
DO - 10.1016/0003-2697(90)90185-C
M3 - Article
C2 - 1705393
AN - SCOPUS:0025133304
SN - 0003-2697
VL - 190
SP - 233
EP - 237
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 2
ER -