An immunological method for quantifying antibacterial activity in Salmo salar (Linnaeus, 1758) skin mucus

Edgar Narvaez; Jorge Berendsen; Fanny Guzmán; José A A. Gallardo; Luis Mercado

doi:10.1016/j.fsi.2009.10.004

An immunological method for quantifying antibacterial activity in Salmo salar (Linnaeus, 1758) skin mucus

Edgar Narvaez, Jorge Berendsen, Fanny Guzmán, José A A. Gallardo, Luis Mercado

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25 Scopus citations

Abstract

Antimicrobial peptides (AMPs) are a pivotal component of innate immunity in lower vertebrates. The aim of this study was to develop an immunological method for quantifying AMPs in Salmo salar skin mucus. A known antimicrobial peptide derived from histone H1 previously purified and described from S. salar skin mucus (SAMP H1) was chemically synthesized and used to obtain antibodies for the quantification of the molecule via ELISA. Using skin mucus samples, a correlation of bacterial growth inhibition versus SAMP H1 concentration (ELISA) was established. The results provide the first evidence for quantifying the presence of active AMPs in the skin mucus of S. salar through the use of an immunological method.

Original language	English
Pages (from-to)	235-239
Number of pages	5
Journal	Fish and Shellfish Immunology
Volume	28
Issue number	1
DOIs	https://doi.org/10.1016/j.fsi.2009.10.004
State	Published - Jan 2010

Keywords

Antibacterial quantification
Fish antibacterial peptide
SAMP H1
Salmo salar

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10.1016/j.fsi.2009.10.004

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abstract = "Antimicrobial peptides (AMPs) are a pivotal component of innate immunity in lower vertebrates. The aim of this study was to develop an immunological method for quantifying AMPs in Salmo salar skin mucus. A known antimicrobial peptide derived from histone H1 previously purified and described from S. salar skin mucus (SAMP H1) was chemically synthesized and used to obtain antibodies for the quantification of the molecule via ELISA. Using skin mucus samples, a correlation of bacterial growth inhibition versus SAMP H1 concentration (ELISA) was established. The results provide the first evidence for quantifying the presence of active AMPs in the skin mucus of S. salar through the use of an immunological method.",

keywords = "Antibacterial quantification, Fish antibacterial peptide, SAMP H1, Salmo salar",

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AU - Mercado, Luis

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AB - Antimicrobial peptides (AMPs) are a pivotal component of innate immunity in lower vertebrates. The aim of this study was to develop an immunological method for quantifying AMPs in Salmo salar skin mucus. A known antimicrobial peptide derived from histone H1 previously purified and described from S. salar skin mucus (SAMP H1) was chemically synthesized and used to obtain antibodies for the quantification of the molecule via ELISA. Using skin mucus samples, a correlation of bacterial growth inhibition versus SAMP H1 concentration (ELISA) was established. The results provide the first evidence for quantifying the presence of active AMPs in the skin mucus of S. salar through the use of an immunological method.

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An immunological method for quantifying antibacterial activity in Salmo salar (Linnaeus, 1758) skin mucus

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