TY - JOUR
T1 - An in vitro study of the protective effect of caffeic acid on human erythrocytes
AU - Colina, José R.
AU - Suwalsky, Mario
AU - Manrique-Moreno, Marcela
AU - Petit, Karla
AU - AGUILAR CAVALLO, LUIS FELIPE
AU - Jemiola-Rzeminska, Malgorzata
AU - Strzalka, Kazimierz
N1 - Funding Information:
To Graduate Program in Chemistry for a scholarship to J.R.C., and FONDECYT (research project 1130043 ). Calorimetric measurements were carried out using the instrument purchased thanks to financial support of European Regional Development Fund (contract No. POIG.02.01.00-12-167/08 , project Malopolska Centre of Biotechnology ). The Jagiellonian University is a partner of the Leading National Research Center (KNOW) supported by the Ministry of Science and Higher Education. The authors thank Fernando Neira for his technical assistance and Pablo Zambrano for his contributions to the review of the manuscript.
Funding Information:
To Graduate Program in Chemistry for a scholarship to J.R.C., and FONDECYT (research project 1130043). Calorimetric measurements were carried out using the instrument purchased thanks to financial support of European Regional Development Fund (contract No. POIG.02.01.00-12-167/08, project Malopolska Centre of Biotechnology). The Jagiellonian University is a partner of the Leading National Research Center (KNOW) supported by the Ministry of Science and Higher Education. The authors thank Fernando Neira for his technical assistance and Pablo Zambrano for his contributions to the review of the manuscript.
Publisher Copyright:
© 2018 Elsevier Inc.
Copyright:
Copyright 2019 Elsevier B.V., All rights reserved.
PY - 2019/2/15
Y1 - 2019/2/15
N2 - The interaction and protective effect of caffeic acid (CA) on human erythrocytes (RBC) and molecular models of its membrane were studied. The latter consisted of bilayers built up of dimyristoylphosphatidylcholine (DMPC) and dimyristoylphosphatidylethanolamine (DMPE), representative of phospholipid classes located in the outer and inner monolayers of the human erythrocyte membrane, respectively. X-ray diffraction and differential scanning calorimetry results indicated that CA induced structural and thermotropic perturbations in multilayers and vesicles of DMPC. Fluorescence spectroscopy analysis showed that CA increased the fluidity of DMPC vesicles and of human erythrocyte ghosts. Scanning electron microscopy observations displayed that CA induced morphological alterations to RBC from their normal discoid form to echinocytes. The assessment of its protective capacity showed that CA inhibits RBC morphological alterations and lysis induced by HClO. These findings imply that CA molecules were located in the outer monolayer of the erythrocyte membrane, and that this preferential location might effectively protect the red cells from damage caused by oxidizing species.
AB - The interaction and protective effect of caffeic acid (CA) on human erythrocytes (RBC) and molecular models of its membrane were studied. The latter consisted of bilayers built up of dimyristoylphosphatidylcholine (DMPC) and dimyristoylphosphatidylethanolamine (DMPE), representative of phospholipid classes located in the outer and inner monolayers of the human erythrocyte membrane, respectively. X-ray diffraction and differential scanning calorimetry results indicated that CA induced structural and thermotropic perturbations in multilayers and vesicles of DMPC. Fluorescence spectroscopy analysis showed that CA increased the fluidity of DMPC vesicles and of human erythrocyte ghosts. Scanning electron microscopy observations displayed that CA induced morphological alterations to RBC from their normal discoid form to echinocytes. The assessment of its protective capacity showed that CA inhibits RBC morphological alterations and lysis induced by HClO. These findings imply that CA molecules were located in the outer monolayer of the erythrocyte membrane, and that this preferential location might effectively protect the red cells from damage caused by oxidizing species.
KW - Antioxidant
KW - Caffeic acid
KW - Erythrocyte membrane
KW - Lipid bilayer
UR - http://www.scopus.com/inward/record.url?scp=85057712560&partnerID=8YFLogxK
U2 - 10.1016/j.abb.2018.12.006
DO - 10.1016/j.abb.2018.12.006
M3 - Article
C2 - 30529104
AN - SCOPUS:85057712560
VL - 662
SP - 75
EP - 82
JO - Archives of Biochemistry and Biophysics
JF - Archives of Biochemistry and Biophysics
SN - 0003-9861
ER -