Antenatal betamethasone produces protracted changes in anxiety-like behaviors and in the expression of microtubule-associated protein 2, brain-derived neurotrophic factor and the tyrosine kinase B receptor in the rat cerebellar cortex

Rodrigo Pascual, Martina Valencia, CARLOS EDUARDO FRANCISCO BUSTAMANTE VALDES

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Using classic Golgi staining methods, we previously showed that the administration of synthetic glucocorticoid betamethasone in equivalent doses to those given in cases of human premature birth generates long-term alterations in Purkinje cell dendritic development in the cerebellar cortex. In the present study, we evaluated whether betamethasone alters the immunohistochemical expression of proteins that participate in cerebellar Purkinje cell dendritic development and maintenance, including microtubule-associated protein 2 (MAP2), brain-derived neurotrophic factor (BDNF) and the tyrosine kinase B receptor (TrkB), which are located predominantly in the cerebellar molecular layer where Purkinje cell dendritogenesis occurs. Consistent with our previous Golgi stain studies, we observed that animals prenatally exposed to a single course of betamethasone showed long-term alterations in the expression of MAP2, BDNF and TrkB. Additionally, these protracted molecular changes were accompanied by anxiety-like behaviors in the elevated plus maze and marble burying tests.

Original languageEnglish
Pages (from-to)78-85
Number of pages8
JournalInternational Journal of Developmental Neuroscience
Volume43
DOIs
StatePublished - 1 Jun 2015

Keywords

  • Anxiety-like behavior
  • BDNF
  • Betamethasone
  • Cerebellar cortex
  • MAP2
  • TrkB

Fingerprint

Dive into the research topics of 'Antenatal betamethasone produces protracted changes in anxiety-like behaviors and in the expression of microtubule-associated protein 2, brain-derived neurotrophic factor and the tyrosine kinase B receptor in the rat cerebellar cortex'. Together they form a unique fingerprint.

Cite this