Antimony(III) induces fibroblast-like phenotype, profibrotic factors and reactive oxygen species in mouse renal cells

The aim of this work was to evaluate the effect of Sb^III on cell integrity, expression of profibrotic factors and reactive oxygen species (ROS) in mouse cortical collecting duct cells (M-1 cell line). M-1 cells were incubated with Sb^III for 24 h. Cell morphology and RNA expression level (connecting tissue growth factor, CTGF), α-SMOOTH MUSCLE ACTIN (α-SMA) and collagen I were analysed. The total Sb content according to each cell compartment was determined and ROS production was measured. Eighty percent of the total Sb was detected in the extracellular medium. A significant increase in ROS production and CTGF protein expression were observed at 100 μg L^-1 Sb^III. M-1 cells showed a non-classic epithelial cell shape at 100 μg L^-1 and a reduction in the collecting duct-specific marker aquaporin-2. At 100 μg L^-1, the number of collagen I-positive cells increased. At 300 μg L^-1, a gross cell nuclear rupture was observed. These results demonstrate that an Sb^III concentration of 100 μg L^-1 is able to promote the induction of CTGF and collagen I along with the induction of ROS, which suggests a cytotoxicity of Sb^III in M-1 kidney collecting duct cells.