Application of a new model based on oxygen balance to determine the oxygen uptake rate in mammalian cell chemostat cultures

Yandi Rigual-González, Lucía Gómez, Jonathan Núñez, Mauricio Vergara, ALVARO ENRIQUE DIAZ BARRERA, JULIO ANDRES BERRIOS ARAYA, CLAUDIA VICTORIA ALTAMIRANO GOMEZ

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

The application of dynamic methods for determining the oxygen uptake rate (OUR) in continuous mammalian cell cultures frequently ignores contributions to the oxygen balance such as the oxygen content in the culture medium inlet and outlet and the oxygen transfer between the culture and the headspace through the culture surface. We develop a mathematical model that allows OUR determination in mammalian cell chemostat cultures through a dynamic method, incorporating these neglected variables, as well as the application and validation of this model at two culture temperatures, 37 °C and 33 °C.The proposed model was compared with a model that only includes the OUR term (typical model). The standard error of the proposed model was less than that of the typical model, making the proposed model more accurate. The results showed that at 37 °C, the results significantly differed depending on which model was used. At 33 and 37 °C, the specific oxygen uptake rate (qO2) values obtained with the proposed model were within the range usually reported for mammalian cells. It was also shown that the OUR value was underestimated if the oxygen transfer through the headspace-medium interface was not considered under mild hypothermia conditions. The model could be applied for developing strategies based on OUR monitoring and controlling for recombinant protein production under conditions of mild hypothermia.

Original languageEnglish
Pages (from-to)586-590
Number of pages5
JournalChemical Engineering Science
Volume152
DOIs
StatePublished - 2 Oct 2016

Keywords

  • Dynamic method
  • Ka
  • Mammalian cells
  • Mathematical model for OUR determination

Fingerprint Dive into the research topics of 'Application of a new model based on oxygen balance to determine the oxygen uptake rate in mammalian cell chemostat cultures'. Together they form a unique fingerprint.

Cite this