Arachidonic acid triggers [Ca2+]i increases in rat round spermatids by a likely GPR activation, ERK signalling & ER/acidic compartments Ca2+release

Joaquin Paillamanque, Ana Sanchez-Tusie, Emerson M. Carmona, Claudia L. Treviño, Carolina Sandoval, Francisco Nualart, NELSON EDUARDO OSSES RIVERA, Juan G. Reyes

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Arachidonic acid (AA), a compound secreted by Sertoli cells (SC) in a FSH-dependent manner, is able to induce the release of Ca2+from internal stores in round spermatids and pachytene spermatocytes. In this study, the possible site(s) of action of AA in round spermatids, the signalling pathways associated and the intracellular Ca2+stores targeted by AA-induced signalling were pharmacologically characterized by measuring intracellular Ca2+using fluorescent Ca2+probes. Our results suggest that AA acts by interacting with a fatty acid G protein coupled receptor, initiating a G protein signalling cascade that may involve PLA2 and ERK activation, which in turn opens intracellular ryanodine-sensitive channels as well as NAADP-sensitive channels in acidic intracellular Ca2+stores. The results presented here also suggest that AMPK and PKA modulate this AA-induced Ca2+release from intracellular Ca2+stores in round spermatids. We propose that unsaturated free fatty acid lipid signalling in the seminiferous tubule is a novel regulatory component of rat spermatogenesis.

Original languageEnglish
Article numbere0172128
JournalPLoS ONE
Volume12
Issue number2
DOIs
StatePublished - 1 Feb 2017

Fingerprint Dive into the research topics of 'Arachidonic acid triggers [Ca<sup>2+]</sup>i increases in rat round spermatids by a likely GPR activation, ERK signalling & ER/acidic compartments Ca<sup>2+</sup>release'. Together they form a unique fingerprint.

Cite this