Beta-glucan enhances the response to SVCV infection in zebrafish

Regla M. Medina-Gali, María del Mar Ortega-Villaizan, Luis Mercado, Beatriz Novoa, Julio Coll, Luis Perez

Research output: Contribution to journalArticlepeer-review

42 Scopus citations


The antiviral effects of beta-glucan, an immunostimulatory agent were studied in zebrafish both in vitro and in vivo. Here we show that zebrafish ZF4 cells as well as whole fish primed with yeast β-glucan zymosan exhibited increased cytokine expression and elevated response to spring viremia of carp virus (SVCV) infection. In vitro, previous treatment of β-glucan enhanced ZF4 cell viability against SVCV infection which is associated to the activation of interferon signaling pathway and inflammatory cytokines gene expression. In vivo, the SVCV-infected fish primed with β-glucan had a higher survival rate (≈73%) than the control SVCV-infected group (≈33%). Additionally, up-regulation of the expression of a set of genes involved in innate immune response was detected in zebrafish intraperitoneally injected of β-glucan: il1b, il6, il8, il10 and tnfa transcripts showed increased expression that appear to be rapid (2 days) but not long-lived (less than 2 weeks). The present study is, to our knowledge, the first to combine cell culture and in vivo approaches to describe host response to β-glucan stimulation and viral infection in zebrafish.

Original languageEnglish
Pages (from-to)307-314
Number of pages8
JournalDevelopmental and Comparative Immunology
StatePublished - Jul 2018


  • Antiviral
  • Beta-glucan
  • Immunostimulant
  • SVCV
  • Zebrafish


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