Bio-hydrogen production during acidogenic fermentation in a multistage stirred tank reactor

Estela Tapia-Venegas, Juan Esteban Ramirez, Andrés Donoso-Bravo, Lorena Jorquera, Jean Phillipe Steyer, Gonzalo Ruiz-Filippi

Research output: Contribution to journalArticlepeer-review

28 Scopus citations


The objective of this study was to evaluate the production of hydrogen in a two-stage CSTR system - both reactors having the same volume - and compare its performance with a conventional one-stage process. The lab-scale two-stage and one-stage systems were operated at five pHs and five hydraulic retention time (HRTs). The maximum volumetric hydrogen productivity and yield obtained with the two-stage system were 5.8 μmol L-1 h-1 and 2.7 mol H2 mol glucose-1, respectively, at an HRT of 12 h and pH 5.5. Overall, the twostage system showed, at steady state, a better performance that the one-stage system for all the evaluated pHs. However, a comparison between the one-stage system, operating at 6 h of HRT, and the first reactor of the two-stage system at the same HRT did not show any significant difference, highlighting the positive impact of having a two-stage process. The determination of the ratio between the experimental measured H2 in the gas phase and the theoretical H2 generated in the liquid phase (discrepancy factor) indicated that an important part of the hydrogen produced in the first reactor was transferred into the second reactor instead of being desorbed in the headspace. Therefore, the improving of hydrogen production in the two-stage system is rather attributed to the increased transfer of hydrogen from liquid to gas than an actual total hydrogen production increase.

Original languageEnglish
Pages (from-to)2185-2190
Number of pages6
JournalInternational Journal of Hydrogen Energy
Issue number5
StatePublished - 19 Feb 2013


  • Anaerobic digestion
  • Bio-hydrogen
  • Plug flow
  • Renewable energy source
  • Series reactor


Dive into the research topics of 'Bio-hydrogen production during acidogenic fermentation in a multistage stirred tank reactor'. Together they form a unique fingerprint.

Cite this