TY - JOUR
T1 - Biochemical profile of leaf, silk and grain samples of eight maize landraces (Zea Mays L.) cultivated in two low-input agricultural systems
AU - Uarrota, Virgílio Gavicho
AU - Severino, Ricardo Brasil
AU - Malinowsky, Carina
AU - de Oliveira, Simone Kobe
AU - Kuhnen, Shirley
AU - Yunes, Rosendo Augusto
AU - Maraschin, Marcelo
N1 - Publisher Copyright:
© 2014 Wiley Periodicals, Inc.
PY - 2014/12/1
Y1 - 2014/12/1
N2 - This research aimed to determine the biochemical profile of leaf, silk and grain samples of eight maize landraces (Zea maysL.) cultivated in southern Brazil. To accomplish this, reverse-phase high performance liquid chromatography-UV-visible (RP-HPLC-UV-vis), UV-vis spectrophotometry, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and chemometrics were used to examine carotenoids and their isomers, anthocyanin and phenolic acids. Leaf tissue samples showed higher amounts of carotenoids (838.6μg/g - Língua de Papagaio variety), anthocyanins (405.8μg/g - Palha Roxa variety) and phenolics (655.0μg/g - Roxo variety), followed by maize silks and grains. RP-HPLC-UV-vis analysis of grain extracts revealed xanthophylls lutein and zeaxanthin as the major compounds. The anthocyanin profile by MALDI-TOF MS identified pelargonidin, cyanidin, peonidin, malvidin and glucoside derivatives in leaf extracts, and silk samples were shown to contain cyanidin, peonidin and 7-methoxy-apigeninidin glucoside. These findings indicate that the discarded material, i.e., leaves and silks, can be prospected as bioactive biomasses useful to industrial sectors.
AB - This research aimed to determine the biochemical profile of leaf, silk and grain samples of eight maize landraces (Zea maysL.) cultivated in southern Brazil. To accomplish this, reverse-phase high performance liquid chromatography-UV-visible (RP-HPLC-UV-vis), UV-vis spectrophotometry, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and chemometrics were used to examine carotenoids and their isomers, anthocyanin and phenolic acids. Leaf tissue samples showed higher amounts of carotenoids (838.6μg/g - Língua de Papagaio variety), anthocyanins (405.8μg/g - Palha Roxa variety) and phenolics (655.0μg/g - Roxo variety), followed by maize silks and grains. RP-HPLC-UV-vis analysis of grain extracts revealed xanthophylls lutein and zeaxanthin as the major compounds. The anthocyanin profile by MALDI-TOF MS identified pelargonidin, cyanidin, peonidin, malvidin and glucoside derivatives in leaf extracts, and silk samples were shown to contain cyanidin, peonidin and 7-methoxy-apigeninidin glucoside. These findings indicate that the discarded material, i.e., leaves and silks, can be prospected as bioactive biomasses useful to industrial sectors.
UR - http://www.scopus.com/inward/record.url?scp=84916232320&partnerID=8YFLogxK
U2 - 10.1111/jfbc.12087
DO - 10.1111/jfbc.12087
M3 - Article
AN - SCOPUS:84916232320
SN - 0145-8884
VL - 38
SP - 551
EP - 562
JO - Journal of Food Biochemistry
JF - Journal of Food Biochemistry
IS - 6
ER -