Broth medium for the successful culture of the fish pathogen Piscirickettsia salmonis

A. J. Yañez, K. Valenzuela, H. Silva, JULIO ENRIQUE RETAMALES LARA, A. Romero, R. Enriquez, J. Figueroa, A. Claude, J. Gonzalez, R. Avendaño-Herrera, J. G. Carcamo

Research output: Contribution to journalArticlepeer-review

61 Scopus citations

Abstract

Piscirickettsiosis or salmonid rickettsial septicaemia (SRS) caused by Piscirickettsia salmonis constitutes one of the main problems in farmed salmonid and marine fishes. Since the first reports of the disease, it has been successfully isolated and maintained in eukaryotic cellculture systems, but these systems are time-consuming, the media are costly, and eliminating heavily contaminated host cell debris is difficult. In this report, we describe a marine-based broth supplemented with L-cysteine, named AUSTRAL-SRS broth, that facilitates superior growth of P. salmonis strains. Strains reached an optical density of approximately 1.8 when absorbance was measured at 600 nm after 6 d incubation at 18°C. Several passages (n = 6) did not alter the culture kinetics. We report for the first time the purification of DNA, lipopolysaccharide (LPS) and whole membrane protein obtained from P. salmonis grown in this liquid medium, and thus provide a suitable platform to simplify the preparation of P. salmonis cells for genetic and serological studies. Moreover, the results of the cytopathic effect test showed that P. salmonis grown in AUSTRALSRS broth maintained their virulence properties, inducing apoptosis after 3 d. This makes the medium a good candidate for the successful growth of P. salmonis and an excellent basis for the development of low cost vaccines.

Original languageEnglish
Pages (from-to)197-205
Number of pages9
JournalDiseases of Aquatic Organisms
Volume97
Issue number3
DOIs
StatePublished - 24 Jan 2012

Keywords

  • Broth
  • Culture
  • Piscirickettsia salmonis

Fingerprint

Dive into the research topics of 'Broth medium for the successful culture of the fish pathogen Piscirickettsia salmonis'. Together they form a unique fingerprint.

Cite this