Co-immobilized β-galactosidase and Saccharomyces cerevisiae cells for the simultaneous synthesis and purification of galacto-oligosaccharides

Carla Aburto, Cecilia Guerrero, Carlos Vera, Lorena Wilson, Andrés Illanes

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Simultaneous synthesis and purification (SSP) of galacto-oligosaccharides (GOS) from lactose was conducted using a combi-biocatalyst formed by crosslinked enzyme aggregates of Aspergillus oryzae β-galactosidase and Saccharomyces cerevisiae cells co-immobilized by entrapment in calcium alginate gel particles. Product yield obtained with the combi-biocatalyst was similar than obtained with the soluble enzyme (23.3%), having a final purity of 25.7%. During the simultaneous process, ethyl-β-galactoside was produced from the ethanol generated as a metabolic product of yeast cells, but ethyl-β-galactoside was considerably decreased at high aeration (4 vvm). The combi-biocatalyst can be recovered and reused but its performance is limited by the reduction of the metabolic capacity of the cells. In this way, a process was developed for the SSP of GOS from lactose, obtaining a comparable product yield and higher specific productivity than in a conventional synthesis.

Original languageEnglish
Pages (from-to)102-108
Number of pages7
JournalEnzyme and Microbial Technology
Volume118
DOIs
StatePublished - Nov 2018
Externally publishedYes

Keywords

  • Ethyl-β-galactoside
  • GOS purification
  • Galacto-oligosaccharides
  • β-galactosidase

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