TY - JOUR
T1 - Continuous biohydrogen production by a degenerated strain of Clostridium acetobutylicum ATCC 824
AU - Guerrero, Karlo
AU - Gallardo, Roberto
AU - Paredes, Iván
AU - Quintero, Julián
AU - Mau, Silvia
AU - CONEJEROS RISCO, RAUL JORGE
AU - Gentina, Juan Carlos
AU - AROCA ARCAYA, GERMAN EDUARDO
N1 - Publisher Copyright:
© 2020 Hydrogen Energy Publications LLC
Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2021/1/27
Y1 - 2021/1/27
N2 - Degenerated strains of Clostridium acetobutylicum lack the ability to produce solvents and to sporulate, allowing the continuous production of hydrogen and organic acids. A degenerated strain of Clostridium acetobutylicum was obtained through successive batch cultures. Its kinetic characterization showed a similar specific growth rate than the wild type (0.25 h−1), a higher butyric acid production of 6.8 g·L−1 and no solvents production. A steady state was reached in a continuous culture at a dilution rate of 0.1 h−1, with a constant hydrogen production of 507 mL·h−1, corresponding to a volumetric rate of 6.10 L·L−1 d−1, and a yield of 2.39 mol of H2 per mole of glucose which represents 60% of the theoretical maximum yield. These results suggest that the degeneration is an interesting alternative for hydrogen production with this strain, obtaining a high hydrogen production in a continuous culture with cells in a permanent acidogenic state.
AB - Degenerated strains of Clostridium acetobutylicum lack the ability to produce solvents and to sporulate, allowing the continuous production of hydrogen and organic acids. A degenerated strain of Clostridium acetobutylicum was obtained through successive batch cultures. Its kinetic characterization showed a similar specific growth rate than the wild type (0.25 h−1), a higher butyric acid production of 6.8 g·L−1 and no solvents production. A steady state was reached in a continuous culture at a dilution rate of 0.1 h−1, with a constant hydrogen production of 507 mL·h−1, corresponding to a volumetric rate of 6.10 L·L−1 d−1, and a yield of 2.39 mol of H2 per mole of glucose which represents 60% of the theoretical maximum yield. These results suggest that the degeneration is an interesting alternative for hydrogen production with this strain, obtaining a high hydrogen production in a continuous culture with cells in a permanent acidogenic state.
KW - Biohydrogen
KW - Clostridium acetobutylicum
KW - Dark fermentation
KW - Degenerated strain
KW - Hydrogen production
UR - http://www.scopus.com/inward/record.url?scp=85097682193&partnerID=8YFLogxK
U2 - 10.1016/j.ijhydene.2020.11.104
DO - 10.1016/j.ijhydene.2020.11.104
M3 - Article
AN - SCOPUS:85097682193
VL - 46
SP - 5100
EP - 5111
JO - International Journal of Hydrogen Energy
JF - International Journal of Hydrogen Energy
SN - 0360-3199
IS - 7
ER -