Continuous biohydrogen production by a degenerated strain of Clostridium acetobutylicum ATCC 824

Karlo Guerrero, Roberto Gallardo, Iván Paredes, Julián Quintero, Silvia Mau, Raúl Conejeros, Juan Carlos Gentina, German Aroca

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7 Scopus citations


Degenerated strains of Clostridium acetobutylicum lack the ability to produce solvents and to sporulate, allowing the continuous production of hydrogen and organic acids. A degenerated strain of Clostridium acetobutylicum was obtained through successive batch cultures. Its kinetic characterization showed a similar specific growth rate than the wild type (0.25 h−1), a higher butyric acid production of 6.8 g·L−1 and no solvents production. A steady state was reached in a continuous culture at a dilution rate of 0.1 h−1, with a constant hydrogen production of 507 mL·h−1, corresponding to a volumetric rate of 6.10 L·L−1 d−1, and a yield of 2.39 mol of H2 per mole of glucose which represents 60% of the theoretical maximum yield. These results suggest that the degeneration is an interesting alternative for hydrogen production with this strain, obtaining a high hydrogen production in a continuous culture with cells in a permanent acidogenic state.

Original languageEnglish
Pages (from-to)5100-5111
Number of pages12
JournalInternational Journal of Hydrogen Energy
Issue number7
StatePublished - 27 Jan 2021


  • Biohydrogen
  • Clostridium acetobutylicum
  • Dark fermentation
  • Degenerated strain
  • Hydrogen production


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