Cryopreservation of european chestnut germplasm

M. C. San-Jose, LORENA VERONICA JORQUERA MARTINEZ, N. Vidal, E. Corredoira, C. Sanchez

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

6 Scopus citations

Abstract

The objective of this study was to develop a cryopreservation method for zygotic embryonic axes, somatic embryos and apices excised from shoot cultures of chestnut. Between 93 and 100% of embryonic axes survived liquid nitrogen (LN) storage following desiccation to moisture contents of 24-20% (on a fresh weight basis), and 63% subsequently developed as whole plants. The ability of desiccation- and vitrification- based procedures enables embryogenic cultures to withstand cryopreservation. A 68% recovery rate was achieved by 3-day pre-culture on high-sucrose medium followed by 60 min application of PVS2 vitrification solution prior to cryogenic storage. A 40-60% recovery rate was obtained when cryostored shoot apices 0.5-1.0 mm long, exposed to PVS2 solution for 120 min at 0°C, were cultured on Gresshoff and Doy (1972) medium supplemented with growth regulators. Cold hardening (1-2 weeks) of the stock material improved shoot regrowth.

Original languageEnglish
Title of host publicationIII International Chestnut Congress
PublisherInternational Society for Horticultural Science
Pages225-232
Number of pages8
ISBN (Print)9789066051003
DOIs
StatePublished - 1 Jan 2005

Publication series

NameActa Horticulturae
Volume693
ISSN (Print)0567-7572

Keywords

  • Castanea sativa
  • Desiccation
  • Embryonic axes
  • Liquid nitrogen
  • Shoot apices
  • Somatic embryos
  • Vitrification

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