Detailed analysis of galactooligosaccharides synthesis with β-galactosidase from Aspergillus oryzae

Paulina Urrutia, Bárbara Rodriguez-Colinas, Lucía Fernandez-Arrojo, Antonio O. Ballesteros, Lorena Wilson, Andrés Illanes, Francisco J. Plou

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The synthesis of galactooligosaccharides (GOS) catalyzed by β-galactosidase from Aspergillus oryzae (Enzeco) was studied. Using 400 g/L of lactose and 15 U/mL, maximum GOS yield, measured by HPAEC-PAD, was 26.8% w/w of total carbohydrates, obtained at approximately 70% lactose conversion. No less than 17 carbohydrates were identified; the major transgalactosylation product was 6′-O-β-galactosyl-lactose, representing nearly one-third (in weight) of total GOS. In contrast with previous reports, the presence of at least five disaccharides was detected, which accounted for 40% of the total GOS at the point of maximum GOS concentration (allolactose and 6-galactobiose were the major products). A. oryzae β-galactosidase showed a preference to form β(1→6) bonds, followed by β(1→3) and β(1→4) linkages. Results were compared with those obtained with β-galactosidases from Kluyveromyces lactis and Bacillus circulans. The highest GOS yield and specific productivity were achieved with B. circulans β-galactosidase. The specificity of the linkages formed and distribution of di-, tri-, and higher GOS varied significantly among the three β-galactosidases.

Original languageEnglish
Pages (from-to)1081-1087
Number of pages7
JournalJournal of Agricultural and Food Chemistry
Issue number5
StatePublished - 6 Feb 2013


  • galactooligosaccharides
  • glycosidase
  • oligosaccharides
  • prebiotics
  • transglycosylation
  • β-galactosidase


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