TY - JOUR
T1 - Detección simultanea de CTV, CEVd y HSVd a través de cidro Arizona 861-S1 y RT-PCR
AU - Camps, RocíO
AU - Castro, MóNica
AU - Besoain, Ximena
N1 - Publisher Copyright:
© 2014 Pontificia Universidad Catolica de Chile, Facultad de Agronomia e Ingenieria Forestal. All rights reserved.
PY - 2014
Y1 - 2014
N2 - Citrus tristeza virus (CTV), Citrus exocortis viroid (CEVd) and Hop stunt viroid (HSVd) are pathogens that affect citrus trees in Chile. The simultaneous use of a rapid diagnostic method with the effective cleaning of infected budwood is a powerful preventative method for controlling viral diseases. The objective of this study was the multiple detection of CTV, CEVd, and HSVd, through bio-amplification in Arizona 861-S1 citron plants, combined with two incubation temperatures and multiple RT-PCR in two steps. Citron indicator plants were inoculated with the three pathogens, both individually and simultaneously, with four un-inoculated plants as controls. After four months of incubation at the two different temperatures, both the multiple and individually inoculated plants showed symptoms associated with these pathogens, which were subsequently corroborated through the use of multiple RT-PCR in two steps. This diagnostic method could be employed as an efficient, cost-effective alternative to the methods that are currently in use for detecting CTV, CEVd and HSVd, nevertheless its effectiveness should also be evaluated with tests using different isolates of these pathogens.
AB - Citrus tristeza virus (CTV), Citrus exocortis viroid (CEVd) and Hop stunt viroid (HSVd) are pathogens that affect citrus trees in Chile. The simultaneous use of a rapid diagnostic method with the effective cleaning of infected budwood is a powerful preventative method for controlling viral diseases. The objective of this study was the multiple detection of CTV, CEVd, and HSVd, through bio-amplification in Arizona 861-S1 citron plants, combined with two incubation temperatures and multiple RT-PCR in two steps. Citron indicator plants were inoculated with the three pathogens, both individually and simultaneously, with four un-inoculated plants as controls. After four months of incubation at the two different temperatures, both the multiple and individually inoculated plants showed symptoms associated with these pathogens, which were subsequently corroborated through the use of multiple RT-PCR in two steps. This diagnostic method could be employed as an efficient, cost-effective alternative to the methods that are currently in use for detecting CTV, CEVd and HSVd, nevertheless its effectiveness should also be evaluated with tests using different isolates of these pathogens.
KW - CEVd
KW - CTV
KW - Citrus
KW - Diagnostic methods
KW - HSVd
KW - Rapid detection
UR - http://www.scopus.com/inward/record.url?scp=84907462861&partnerID=8YFLogxK
U2 - 10.4067/S0718-16202014000200012
DO - 10.4067/S0718-16202014000200012
M3 - Article
AN - SCOPUS:84907462861
VL - 41
SP - 255
EP - 261
JO - Ciencia e Investigacion Agraria
JF - Ciencia e Investigacion Agraria
SN - 0304-5609
IS - 2
ER -