Detection of the hepcidin prepropeptide and mature peptide in liver of rainbow trout

Claudio A. Álvarez; Paula A. Santana; Fanny Guzmán; Sergio Marshall; Luis Mercado

doi:10.1016/j.dci.2013.04.002

Detection of the hepcidin prepropeptide and mature peptide in liver of rainbow trout

Claudio A. Álvarez, Paula A. Santana, Fanny Guzmán, Sergio Marshall, Luis Mercado

INSTITUTE OF BIOLOGY

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

Hepcidin is a small, cationic peptide which displays antimicrobial activities and iron regulatory function. Originally identified in mammals, this peptide is also present in fish. Hepcidin mRNA is predominantly expressed in liver and is regulated by iron and pathogen infection. In this work, we characterized the expression of trout hepcidin at protein level using rabbit antisera. Results showed that the prepropeptide of hepcidin can be detected by Western Blot in liver tissue from trout injected with iron or lipopolysaccharide. The mature hepcidin peptide was detected at the ionized state 5+(m/z 577.2) by HPLC-ESI-MS in acid extracts from liver tissue. Moreover, hepcidin peptide was located in trout liver imprints by immunofluorescence. These results showed that hepcidin peptide is up-regulated by iron and bacterial components in the trout liver. This up-regulation could be a potential indicator of disease susceptibility, suggesting that hepcidin regulates iron homeostasis in salmonids.

Original language	English
Pages (from-to)	77-81
Number of pages	5
Journal	Developmental and Comparative Immunology
Volume	41
Issue number	1
DOIs	https://doi.org/10.1016/j.dci.2013.04.002
State	Published - Sep 2013

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title = "Detection of the hepcidin prepropeptide and mature peptide in liver of rainbow trout",

abstract = "Hepcidin is a small, cationic peptide which displays antimicrobial activities and iron regulatory function. Originally identified in mammals, this peptide is also present in fish. Hepcidin mRNA is predominantly expressed in liver and is regulated by iron and pathogen infection. In this work, we characterized the expression of trout hepcidin at protein level using rabbit antisera. Results showed that the prepropeptide of hepcidin can be detected by Western Blot in liver tissue from trout injected with iron or lipopolysaccharide. The mature hepcidin peptide was detected at the ionized state 5+(m/z 577.2) by HPLC-ESI-MS in acid extracts from liver tissue. Moreover, hepcidin peptide was located in trout liver imprints by immunofluorescence. These results showed that hepcidin peptide is up-regulated by iron and bacterial components in the trout liver. This up-regulation could be a potential indicator of disease susceptibility, suggesting that hepcidin regulates iron homeostasis in salmonids.",

author = "{\'A}lvarez, {Claudio A.} and Santana, {Paula A.} and Fanny Guzm{\'a}n and Sergio Marshall and Luis Mercado",

note = "Funding Information: This work was funded by a doctoral thesis grant 24110237 from CONICYT Chile and Regular Project 037.418/2012 from the Direcci{\'o}n de Investigaci{\'o}n, Pontificia Universidad Cat{\'o}lica de Valpara{\'i}so. CA and PS are fellows of Advanced Human Capital Formation of CONICYT, Chile. We thank Ms. Drew Neavin and Paulina Schmitt PhD for their English review assistance. ",

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doi = "10.1016/j.dci.2013.04.002",

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AU - Santana, Paula A.

AU - Guzmán, Fanny

AU - Marshall, Sergio

AU - Mercado, Luis

N1 - Funding Information: This work was funded by a doctoral thesis grant 24110237 from CONICYT Chile and Regular Project 037.418/2012 from the Dirección de Investigación, Pontificia Universidad Católica de Valparaíso. CA and PS are fellows of Advanced Human Capital Formation of CONICYT, Chile. We thank Ms. Drew Neavin and Paulina Schmitt PhD for their English review assistance.

PY - 2013/9

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N2 - Hepcidin is a small, cationic peptide which displays antimicrobial activities and iron regulatory function. Originally identified in mammals, this peptide is also present in fish. Hepcidin mRNA is predominantly expressed in liver and is regulated by iron and pathogen infection. In this work, we characterized the expression of trout hepcidin at protein level using rabbit antisera. Results showed that the prepropeptide of hepcidin can be detected by Western Blot in liver tissue from trout injected with iron or lipopolysaccharide. The mature hepcidin peptide was detected at the ionized state 5+(m/z 577.2) by HPLC-ESI-MS in acid extracts from liver tissue. Moreover, hepcidin peptide was located in trout liver imprints by immunofluorescence. These results showed that hepcidin peptide is up-regulated by iron and bacterial components in the trout liver. This up-regulation could be a potential indicator of disease susceptibility, suggesting that hepcidin regulates iron homeostasis in salmonids.

AB - Hepcidin is a small, cationic peptide which displays antimicrobial activities and iron regulatory function. Originally identified in mammals, this peptide is also present in fish. Hepcidin mRNA is predominantly expressed in liver and is regulated by iron and pathogen infection. In this work, we characterized the expression of trout hepcidin at protein level using rabbit antisera. Results showed that the prepropeptide of hepcidin can be detected by Western Blot in liver tissue from trout injected with iron or lipopolysaccharide. The mature hepcidin peptide was detected at the ionized state 5+(m/z 577.2) by HPLC-ESI-MS in acid extracts from liver tissue. Moreover, hepcidin peptide was located in trout liver imprints by immunofluorescence. These results showed that hepcidin peptide is up-regulated by iron and bacterial components in the trout liver. This up-regulation could be a potential indicator of disease susceptibility, suggesting that hepcidin regulates iron homeostasis in salmonids.

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Detection of the hepcidin prepropeptide and mature peptide in liver of rainbow trout

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