TY - JOUR
T1 - Determination of kinetic parameters of fermentation processes by a continuous unsteady‐state method
T2 - Application to the alcoholic fermentation of D‐xylose by Pichia stipitis
AU - Domínguez, H.
AU - Núñez, M. N.
AU - Chamy, R.
AU - Lema, J. M.
PY - 1993/5
Y1 - 1993/5
N2 - A quick technique for determination of kinetic parameters of fermentation processes is proposed and applied to the transformation of D‐xylose into ethanol by Pichia stipitis. The commonly used method to evaluate these parameters is based on achieving several steady states. In the proposed procedure, μm and Ks can be determined from only one steady state, by provoking a disturbance over it, after allowing the system to return to the original conditions. The main difference between the steady and unsteady state methods is the required fermentation time; while the former method lasted 350 h, the latter required a period 25 times lower. Kinetic and stoichiometric parameters were determined with both methods under anoxic and limited oxygen concentration conditions. Results from the two methods were compared, giving only 2% and 4.5% differences in the values of Ks and μm and a little over 4% for μm were the deviations under the latter ones. © 1993 Wiley & Sons, Inc.
AB - A quick technique for determination of kinetic parameters of fermentation processes is proposed and applied to the transformation of D‐xylose into ethanol by Pichia stipitis. The commonly used method to evaluate these parameters is based on achieving several steady states. In the proposed procedure, μm and Ks can be determined from only one steady state, by provoking a disturbance over it, after allowing the system to return to the original conditions. The main difference between the steady and unsteady state methods is the required fermentation time; while the former method lasted 350 h, the latter required a period 25 times lower. Kinetic and stoichiometric parameters were determined with both methods under anoxic and limited oxygen concentration conditions. Results from the two methods were compared, giving only 2% and 4.5% differences in the values of Ks and μm and a little over 4% for μm were the deviations under the latter ones. © 1993 Wiley & Sons, Inc.
KW - D‐xylose
KW - Pichia stipitis
KW - kinetic parameters
KW - unsteady state
UR - http://www.scopus.com/inward/record.url?scp=0027604289&partnerID=8YFLogxK
U2 - 10.1002/bit.260411117
DO - 10.1002/bit.260411117
M3 - Letter
C2 - 18601300
AN - SCOPUS:0027604289
VL - 41
SP - 1129
EP - 1132
JO - Biotechnology and Bioengineering
JF - Biotechnology and Bioengineering
SN - 0006-3592
IS - 11
ER -