Determination of minimal concentration of Piscirickettsia salmonis in water columns to establish a fallowing period in salmon farms

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24 Scopus citations

Abstract

A highly sensitive real-time PCR procedure to detect and quantify the number of Pisciricketsia salmonis units in seawater samples from affected farm sites has been developed. The purpose was to determine a fallowing period that would allow safe restocking of the target farm with new fish. Bacterial load was determined in water samples by comparing the obtained amplification values against a standard curve generated by the amplification of known concentrations of the ITS-ribosomal component of P. salmonis DNA, cloned in a suitable vector. The standard curve was linear over the range of 101-1010 log units. Target samples were taken every 10 days over a 40-day period, at 5 m depth and at the surface. In a highly affected area of southern Chile, the number of bacterial units in farm water decreased to zero at day 50. Therefore, a fallowing period of 50 days post-removal of cages of affected fish appears to be appropriate before restocking. This procedure could be adapted to control disease problems because of other pathogens in fish farm waters.

Original languageEnglish
Pages (from-to)261-266
Number of pages6
JournalJournal of Fish Diseases
Volume33
Issue number3
DOIs
StatePublished - 1 Mar 2010

Keywords

  • Bacterial load
  • Fallowing period
  • Piscirickettsia salmonis

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