Determination of the absolute configuration of selenomethionine from antarctic krill by RP-HPLC/ICP-MS using chiral derivatization agents

JAN BERGMANN , Stephan Lassen, Andreas Prange

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

A fast and sensitive method was developed for the determination of the absolute configuration of selenomethionine. The enantiomers of selenomethionine were converted into diastereomeric isoindole derivatives by reaction with o-phthaldialdehyde and N-isobutyryl-l-cysteine. This easy-to-handle reaction proceeds quantitatively in a few minutes at room temperature. Separation and detection of the diastereomers was achieved by reversed-phase high-performance liquid chromatography-inductively coupled plasma-mass spectrometry (RP-HPLC/ICP-MS) using a conventional C18 reversed-phase column. Detection limits of about 4 μg L-1 were obtained. The method was applied to the determination of the configuration of selenomethionine extracted from antarctic krill, which turned out to possess the l-configuration.

Original languageEnglish
Pages (from-to)1624-1629
Number of pages6
JournalAnalytical and Bioanalytical Chemistry
Volume378
Issue number6
DOIs
StatePublished - 1 Mar 2004

Keywords

  • Chiral speciation
  • Enantiomer separation
  • Inductively coupled plasma mass spectrometry
  • Krill
  • Selenomethionine

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