Abstract
A fast and sensitive method was developed for the determination of the absolute configuration of selenomethionine. The enantiomers of selenomethionine were converted into diastereomeric isoindole derivatives by reaction with o-phthaldialdehyde and N-isobutyryl-l-cysteine. This easy-to-handle reaction proceeds quantitatively in a few minutes at room temperature. Separation and detection of the diastereomers was achieved by reversed-phase high-performance liquid chromatography-inductively coupled plasma-mass spectrometry (RP-HPLC/ICP-MS) using a conventional C18 reversed-phase column. Detection limits of about 4 μg L-1 were obtained. The method was applied to the determination of the configuration of selenomethionine extracted from antarctic krill, which turned out to possess the l-configuration.
| Original language | English |
|---|---|
| Pages (from-to) | 1624-1629 |
| Number of pages | 6 |
| Journal | Analytical and Bioanalytical Chemistry |
| Volume | 378 |
| Issue number | 6 |
| DOIs | |
| State | Published - Mar 2004 |
| Externally published | Yes |
Keywords
- Chiral speciation
- Enantiomer separation
- Inductively coupled plasma mass spectrometry
- Krill
- Selenomethionine