Development of useful biomaterial for bone tissue Engineering by incorporating Nano-Copper-Zinc Alloy (nCuZn) in Chitosan/Gelatin/Nano-Hydroxyapatite (Ch/G/nHAp) scaffold

Juan Carlos Forero, Eduardo Roa, Juan G. Reyes, Cristian Acevedo, NELSON EDUARDO OSSES RIVERA

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Ceramic and metallic nanoparticles can improve the mechanical and biological properties of polymeric scaffolds for bone tissue engineering (BTE). In this work, nanohydroxyapatite (nHAp) and nano-copper-zinc alloy (nCuZn) were added to a chitosan/gelatin (Ch/G) scaffold in order to investigate the effects on morphological, physical, and biocompatibility properties. Scaffolds were fabricated by a freeze-drying technique using different pre-freezing temperatures. Microstructure and morphology were studied by scanning electron microscopy (SEM), glass transition (Tg) was studied using differential scanning calorimetry (DSC), cell growth was estimated by MTT assay, and biocompatibility was examined in vitro and in vivo by histochemistry analyses. Scaffolds and nanocomposite scaffolds presented interconnected pores, high porosity, and pore size appropriate for BTE. Tg of Ch/G scaffolds was diminished by nanoparticle inclusion. Mouse embryonic fibroblasts (MEFs) cells loaded in the Ch/G/nHAp/nCuZn nanocomposite scaffold showed suitable behavior, based on cell adhesion, cell growth, alkaline phosphatase (ALP) activity as a marker of osteogenic differentiation, and histological in vitro cross sections. In vivo subcutaneous implant showed granulation tissue formation and new tissue infiltration into the scaffold. The favorable microstructure, coupled with the ability to integrate nanoparticles into the scaffold by freeze-drying technique and the biocompatibility, indicates the potential of this new material for applications in BTE.

Original languageEnglish
Article number1177
JournalMaterials
Volume10
Issue number10
DOIs
StatePublished - 17 Oct 2017

Keywords

  • Bone tissue engineering
  • Chitosan
  • Gelatin
  • Metallic nanoparticles
  • Nanohydroxyapatite
  • Scaffold

Fingerprint Dive into the research topics of 'Development of useful biomaterial for bone tissue Engineering by incorporating Nano-Copper-Zinc Alloy (nCuZn) in Chitosan/Gelatin/Nano-Hydroxyapatite (Ch/G/nHAp) scaffold'. Together they form a unique fingerprint.

Cite this