Differential expression and localization of ADAM10 and ADAM17 during rat spermatogenesis suggest a role in germ cell differentiation

Paulina Urriola-Muñoz, Carlos Lizama, Raúl Lagos-Cabré, JUAN GUILLERMO REYES MARTINEZ, Ricardo D. Moreno

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Background: Extracellular metolloproteases have been implied in different process such as cell death, differentiation and migration. Membrane-bound metalloproteases of the ADAM family shed the extracellular domain of many cytokines and receptor controlling auto and para/juxtacrine cell signaling in different tissues. ADAM17 and ADAM10 are two members of this family surface metalloproteases involved in germ cell apoptosis during the first wave of spermatogenesis in the rat, but they have other signaling functions in somatic tissues. Results: In an attempt to further study these two enzymes, we describe the presence and localization in adult male rats. Results showed that both enzymes are detected in germ and Sertoli cells during all the stages of spermatogenesis. Interestingly their protein levels and cell surface localization in adult rats were stage-specific, suggesting activation of these enzymes at particular events of rat spermatogenesis. Conclusions: Therefore, these results show that ADAM10 and ADAM17 protein levels and subcellular (cell surface) localization are regulated during rat spermatogenesis.

Original languageEnglish
Article number31
JournalBiological Research
Volume47
Issue number1
DOIs
StatePublished - 2014
Externally publishedYes

Keywords

  • Apoptosis
  • Metalloproteinase
  • Paracrine
  • Spermatogenesis
  • Testis

Fingerprint Dive into the research topics of 'Differential expression and localization of ADAM10 and ADAM17 during rat spermatogenesis suggest a role in germ cell differentiation'. Together they form a unique fingerprint.

Cite this