DNA methylation in Trypanosoma cruzi

M. Véronica Rojas; Norbel Galanti

doi:10.1016/0014-5793(90)80717-W

DNA methylation in Trypanosoma cruzi

M. Véronica Rojas, Norbel Galanti

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

DNA isolated from the protozoan Trypanosoma cruzi has been found to contain 5-methylcytosine. Analysis of T. cruzi DNA by both HpaII and MspI restriction endonucleases suggests that the sequence -CCGG- is not methylated. Probably T. cruzi DNA also contains N⁶-methyladenine. This report constitutes the first clear demonstration of the presence of methylated bases in the nuclear DNA from trypanosomes.

Original language	English
Pages (from-to)	113-116
Number of pages	4
Journal	FEBS Letters
Volume	263
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(90)80717-W
State	Published - 9 Apr 1990
Externally published	Yes

Keywords

5-Methylcytosine
Base composition
DNA methylation
Trypanosoma cruzi

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10.1016/0014-5793(90)80717-W

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abstract = "DNA isolated from the protozoan Trypanosoma cruzi has been found to contain 5-methylcytosine. Analysis of T. cruzi DNA by both HpaII and MspI restriction endonucleases suggests that the sequence -CCGG- is not methylated. Probably T. cruzi DNA also contains N6-methyladenine. This report constitutes the first clear demonstration of the presence of methylated bases in the nuclear DNA from trypanosomes.",

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author = "Rojas, {M. V{\'e}ronica} and Norbel Galanti",

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AU - Galanti, Norbel

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N2 - DNA isolated from the protozoan Trypanosoma cruzi has been found to contain 5-methylcytosine. Analysis of T. cruzi DNA by both HpaII and MspI restriction endonucleases suggests that the sequence -CCGG- is not methylated. Probably T. cruzi DNA also contains N6-methyladenine. This report constitutes the first clear demonstration of the presence of methylated bases in the nuclear DNA from trypanosomes.

AB - DNA isolated from the protozoan Trypanosoma cruzi has been found to contain 5-methylcytosine. Analysis of T. cruzi DNA by both HpaII and MspI restriction endonucleases suggests that the sequence -CCGG- is not methylated. Probably T. cruzi DNA also contains N6-methyladenine. This report constitutes the first clear demonstration of the presence of methylated bases in the nuclear DNA from trypanosomes.

KW - 5-Methylcytosine

KW - Base composition

KW - DNA methylation

KW - Trypanosoma cruzi

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DNA methylation in Trypanosoma cruzi

Abstract

Keywords

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