TY - JOUR
T1 - Draft genomes and reference transcriptomes extend the coding potential of the fish pathogen Piscirickettsia salmonis
AU - Millar, Angela D.
AU - Tapia, Paz
AU - Gómez, Fernando A.
AU - Marshall, Sergio H.
AU - Fuentes, Derie E.
AU - Valdes, Jorge H.
N1 - Funding Information:
This work was supported by InnovaChile-CORFO ( FCR-CSB 09CEII-6991 ) grant to D.E.F., S.H.M. and J.H.V.; FIE V014 to S.H.M. and F.A.G.; FONDECYT INICIACION 11110434 to J.H.V. and FONDECYT 1170342 to S.H.M.; CONICYT National PhD Scholarship and CONICYT-PAI 7813110018 to A.D.M.
Publisher Copyright:
© 2018
PY - 2018/5
Y1 - 2018/5
N2 - Background: Draft and complete genome sequences from bacteria are key tools to understand genetic determinants involved in pathogenesis in several disease models. Piscirickettsia salmonis is a Gram-negative bacterium responsible for the Salmon Rickettsial Syndrome (SRS), a bacterial disease that threatens the sustainability of the Chilean salmon industry. In previous reports, complete and draft genome sequences have been generated and annotated. However, the lack of transcriptome data underestimates the genetic potential, does not provide information about transcriptional units and contributes to disseminate annotation errors. Results: Here we present the draft genome and transcriptome sequences of four P. salmonis strains. We have identified the transcriptional architecture of previously characterized virulence factors and trait-specific genes associated to cation uptake, metal efflux, antibiotic resistance, secretion systems and other virulence factors. Conclusions: This data has provided a refined genome annotation and also new insights on the transcriptional structures and coding potential of this fish pathogen. Millar AD, Tapia P, Gomez FA, et al. Draft genomes and reference transcriptomes extend the coding potential of the fish pathogen Piscirickettsia salmonis. Electron J Biotechnol 2018;33. https://doi.org/10.1016/j.ejbt.2018.04.002.
AB - Background: Draft and complete genome sequences from bacteria are key tools to understand genetic determinants involved in pathogenesis in several disease models. Piscirickettsia salmonis is a Gram-negative bacterium responsible for the Salmon Rickettsial Syndrome (SRS), a bacterial disease that threatens the sustainability of the Chilean salmon industry. In previous reports, complete and draft genome sequences have been generated and annotated. However, the lack of transcriptome data underestimates the genetic potential, does not provide information about transcriptional units and contributes to disseminate annotation errors. Results: Here we present the draft genome and transcriptome sequences of four P. salmonis strains. We have identified the transcriptional architecture of previously characterized virulence factors and trait-specific genes associated to cation uptake, metal efflux, antibiotic resistance, secretion systems and other virulence factors. Conclusions: This data has provided a refined genome annotation and also new insights on the transcriptional structures and coding potential of this fish pathogen. Millar AD, Tapia P, Gomez FA, et al. Draft genomes and reference transcriptomes extend the coding potential of the fish pathogen Piscirickettsia salmonis. Electron J Biotechnol 2018;33. https://doi.org/10.1016/j.ejbt.2018.04.002.
KW - Bacterial genomes
KW - Coding potential
KW - Comparative analysis
KW - Draft genome
KW - Piscirickettsia salmonis
KW - Reference transcriptome
KW - Refined annotation
KW - Salmon Rickettsial Syndrome
KW - Salmonids
UR - http://www.scopus.com/inward/record.url?scp=85046426368&partnerID=8YFLogxK
U2 - 10.1016/j.ejbt.2018.04.002
DO - 10.1016/j.ejbt.2018.04.002
M3 - Article
AN - SCOPUS:85046426368
VL - 33
SP - 36
EP - 38
JO - Electronic Journal of Biotechnology
JF - Electronic Journal of Biotechnology
SN - 0717-3458
ER -