Prenatal stress caused by movement restriction induces changes in the appendicular osseous development of CF-1 mice progeny

Prenatal stress has been associated with alterations in weight and body size, as well as disturbances in the process of developing skeletal ossification, occurring during childbirth and the early stages of life. However, the effect evidence of prenatal stress on bone growth and development during the gestation period has been low; therefore, it is unknown whether these alterations are associated with potential for growth disorders Because of this, the study aims to determine the short-term effects of prenatal stress on the CF- 1 mouse bone structure growth in your date of birth. The female mice were divided randomly in two groups: controlled (n=2) and stressed (n=3). The latter was put under stress by means of movement restriction during the last week of gestation. Second, an evaluation of their gestational development was made, obtaining measurements of their weight. Finally, diaphanization with KOH and staining with Alizarin red was used to measure the length of their appendicular bones and their flat pelvic bones, of 53 P0 mice (25 control; 28 stressed during gestation). The stressed miceÕs body weight (p=0.02) and the length of their appendicular bones (radii, p=0.0011; ulnae, p= 0.0001; humeri, p=0.0001; femorae, p=0.0006; tibiae, p=0.0015) were affected significantly in contrast with the controlled group. On the other hand, there were no significant differences in maternal body weight and length of the mice pelvic bones (isquium, ilium; p>0.05). The prenatal stress by means of movement restriction alters the osseous appendicular morphology of the CF-1 mouse evaluated at birth.