Enzymatic depolymerization of sugar beet pulp: Production and characterization of pectin and pectic-oligosaccharides as a potential source for functional carbohydrates

Research output: Contribution to journalArticlepeer-review

61 Scopus citations

Abstract

In order to obtain solids extracts rich in hemicellulose from sugar beet pulp (SBP), an enzymatic depolymerization process with pectinolytic enzymes was evaluated. Both enzymatic preparations (Rohapect DA6L and Macer8 FJ) contributed to achieve rich fractions of hemicellulose; however Rohapect DA6L always showed a higher release of pectic oligosaccharides (pectin removal yield of 94.9%). The characterization of the obtained solid residue resulted in 30.6% hemicellulose, 25.95% cellulose, 12.1% lignin, 0.83% pectin and 4.86% protein. The total carbohydrates and reduced sugars in the hydrolysate were 143.5 and 5.24. g/L, respectively. The extracted pectin infrared spectrum revealed that it corresponds to low methoxyl pectin, confirmed by titrimetric method (DM: 56%). It can be stated that enzymatic treatment (with commercial pectinases) of SBP can be used in the recovery of valuable by-products, such as pectin oligosaccharides and solid fractions rich in hemicellulose.

Original languageEnglish
Pages (from-to)29-36
Number of pages8
JournalChemical Engineering Journal
Volume192
DOIs
StatePublished - 1 Jun 2012

Keywords

  • Enzymatic depectinization
  • Lignocellulosic residue
  • Non digestibles oligosaccharides
  • Pectic polysaccharides
  • Pectin
  • Pectinases
  • Sugar beet pulp

Fingerprint Dive into the research topics of 'Enzymatic depolymerization of sugar beet pulp: Production and characterization of pectin and pectic-oligosaccharides as a potential source for functional carbohydrates'. Together they form a unique fingerprint.

Cite this