TY - JOUR

T1 - Estimating the product inhibition constant from enzyme kinetic equations using the direct linear plot method in one-stage treatment

AU - Valencia, Pedro L.

AU - Sepúlveda, Bastián

AU - Gajardo, Diego

AU - Astudillo-Castro, Carolina

N1 - Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2020/8

Y1 - 2020/8

N2 - A direct linear plot was applied to estimate kinetic constants using the product’s competitive inhibition equation. The challenge consisted of estimating three kinetic constants, Vmax, Km, and Kp, using two independent variables, substrates, and product concentrations, in just one stage of mathematical treatment. The method consisted of combining three initial reaction rate data and avoiding the use of the same three product concentrations (otherwise, this would result in a mathematical indetermination). The direct linear plot method was highly superior to the least-squares method in terms of accuracy and robustness, even under the addition of error. The direct linear plot method is a reliable and robust method that can be applied to estimate Kp in inhibition studies in pharmaceutical and biotechnological areas.

AB - A direct linear plot was applied to estimate kinetic constants using the product’s competitive inhibition equation. The challenge consisted of estimating three kinetic constants, Vmax, Km, and Kp, using two independent variables, substrates, and product concentrations, in just one stage of mathematical treatment. The method consisted of combining three initial reaction rate data and avoiding the use of the same three product concentrations (otherwise, this would result in a mathematical indetermination). The direct linear plot method was highly superior to the least-squares method in terms of accuracy and robustness, even under the addition of error. The direct linear plot method is a reliable and robust method that can be applied to estimate Kp in inhibition studies in pharmaceutical and biotechnological areas.

KW - Direct linear plot

KW - Distribution-free method

KW - Kinetic constants

KW - Median method

KW - Non-parametric

KW - Product inhibition

UR - http://www.scopus.com/inward/record.url?scp=85088976611&partnerID=8YFLogxK

U2 - 10.3390/catal10080853

DO - 10.3390/catal10080853

M3 - Article

AN - SCOPUS:85088976611

VL - 10

JO - Catalysts

JF - Catalysts

SN - 2073-4344

IS - 8

M1 - 853

ER -