Ethanol affects the function of a neurotransmitter receptor protein without altering the membrane lipid phase

Juan C. Tapia, LUIS FELIPE AGUILAR CAVALLO, Carlos P. Sotomayor, Luis G. Aguayo

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Using patch-clamp and fluorescence techniques we found that ethanol (10-200 mM) potentiated strychnine-sensitive glycine receptors without having detectable effects on lipid order parameters in mouse spinal cord neurons. Hepthanol (0.01-1 mM), in contrast, did not affect the glycine current, but it altered the core and surface of spinal neuron membranes as detected by changes in 1,6-diphenyl-1,3,5-hexatriene (DPH) and Laurdan fluorescence parameters. These findings support the idea that ethanol affects these membrane proteins without changing lipid fluidity. Copyright (C) 1998 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)239-244
Number of pages6
JournalEuropean Journal of Pharmacology
Volume354
Issue number2-3
DOIs
StatePublished - 7 Aug 1998
Externally publishedYes

Keywords

  • Anisotropy
  • Cl current
  • Ethanol
  • Glycine receptor
  • Laurdan
  • Spinal neuron
  • Strychnine

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