Expression analysis of phytochromes A, B and floral integrator genes during the entry and exit of grapevine-buds from endodormancy

Francisco J. Pérez, NATHALIE ALEXANDRA KUHN WEBER, R. Vergara

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

A common molecular regulatory pathway that involves PHYA, PHYB and floral integrator genes CONSTANS (CO), FLOWERING LOCUS T (FT) and SUPRESSOR OF OVEREXPRESSION OF CO1 (SOC1) has been suggested to participate in the regulation of photoperiod dependent processes such as flowering and dormancy. In grapevines (Vitis vinifera L.), decreasing photoperiod and low temperatures trigger the transition of buds into endodormancy (ED), a process that is accompanied by drastic changes in gene expression of VvPHYA and B in leaves. To analyse the relationship of VvPHYA, VvPHYB, and grape homologues of Arabidopsis floral integrator genes VvCO, VvFT, VvMADS8, with ED, a comparative expression analysis of these genes was performed in grapevine-leaves and buds before, during and after the transition of buds into ED. The expression of all the above genes in the bud-tissue, and the fact that photoperiod regulates differently the expression of VvPHYA and B in buds than in leaves, suggests that the bud might be an autonomous or semi-autonomous organ in perceiving and transducing the photoperiod signal. On the other hand, the coordinated down-regulation of VvFT in leaves and buds during the transition of buds into ED, and its subsequent up-regulation following the application of dormancy-breaking compounds, hydrogen cyanamide (HC) and sodium azide, suggests that VvFT could play a key role in stimulating bud-growth by repressing their entry into ED.

Original languageEnglish
Pages (from-to)1659-1666
Number of pages8
JournalJournal of Plant Physiology
Volume168
Issue number14
DOIs
StatePublished - 15 Sep 2011
Externally publishedYes

Keywords

  • Dormancy
  • Floral integrator genes
  • Phytochromes
  • Vitis vinifera

Fingerprint Dive into the research topics of 'Expression analysis of phytochromes A, B and floral integrator genes during the entry and exit of grapevine-buds from endodormancy'. Together they form a unique fingerprint.

Cite this