TY - JOUR
T1 - Expression of ssa-miR-155 during ISAV infection in vitro
T2 - Putative role as a modulator of the immune response in Salmo salar
AU - Salazar, Carolina
AU - Galaz, Martín
AU - Ojeda, Nicolás
AU - MARSHALL GONZALEZ, SERGIO HERNAN
N1 - Funding Information:
This work was supported by the National Commission for Scientific and Technological Research (CONICYT) , Chile [Grant n° 21120203 ].
Publisher Copyright:
© 2021 Elsevier Ltd
PY - 2021/9
Y1 - 2021/9
N2 - Multiple cellular components are involved in pathogen-host interaction during viral infection; in this context, the role of miRNAs have become highly relevant. We assessed the expression of selected miRNAs during an in vitro infection of a Salmo salar cell line with Infectious Salmon Anemia Virus (ISAV), the causative agent of a severe disease by the same name. Salmon orthologs for miRNAs that regulate antiviral responses were measured using RT-qPCR in an in vitro time-course assay. We observed a modulation of specific miRNAs expression, where ssa-miR-155-5p was differentially over-expressed. Using in silico analysis, we identified the putative mRNA targets for ssa-miR-155-5p, finding a high prevalence of hosts immune response-related genes; moreover, several mRNAs involved in the viral infective process were also identified as targets for this miRNA. Our results suggest a relevant role for miR-155-5p in Salmo salar during an ISAV infection as a regulator of the immune response to the virus.
AB - Multiple cellular components are involved in pathogen-host interaction during viral infection; in this context, the role of miRNAs have become highly relevant. We assessed the expression of selected miRNAs during an in vitro infection of a Salmo salar cell line with Infectious Salmon Anemia Virus (ISAV), the causative agent of a severe disease by the same name. Salmon orthologs for miRNAs that regulate antiviral responses were measured using RT-qPCR in an in vitro time-course assay. We observed a modulation of specific miRNAs expression, where ssa-miR-155-5p was differentially over-expressed. Using in silico analysis, we identified the putative mRNA targets for ssa-miR-155-5p, finding a high prevalence of hosts immune response-related genes; moreover, several mRNAs involved in the viral infective process were also identified as targets for this miRNA. Our results suggest a relevant role for miR-155-5p in Salmo salar during an ISAV infection as a regulator of the immune response to the virus.
KW - Immune response
KW - ISAV
KW - miRNA
KW - Salmo salar
UR - http://www.scopus.com/inward/record.url?scp=85107410663&partnerID=8YFLogxK
U2 - 10.1016/j.dci.2021.104109
DO - 10.1016/j.dci.2021.104109
M3 - Article
C2 - 33930457
AN - SCOPUS:85107410663
VL - 122
JO - Developmental and Comparative Immunology
JF - Developmental and Comparative Immunology
SN - 0145-305X
M1 - 104109
ER -