Expression of ssa-miR-155 during ISAV infection in vitro: Putative role as a modulator of the immune response in Salmo salar

Carolina Salazar, Martín Galaz, Nicolás Ojeda, SERGIO HERNAN MARSHALL GONZALEZ

Research output: Contribution to journalArticlepeer-review

Abstract

Multiple cellular components are involved in pathogen-host interaction during viral infection; in this context, the role of miRNAs have become highly relevant. We assessed the expression of selected miRNAs during an in vitro infection of a Salmo salar cell line with Infectious Salmon Anemia Virus (ISAV), the causative agent of a severe disease by the same name. Salmon orthologs for miRNAs that regulate antiviral responses were measured using RT-qPCR in an in vitro time-course assay. We observed a modulation of specific miRNAs expression, where ssa-miR-155-5p was differentially over-expressed. Using in silico analysis, we identified the putative mRNA targets for ssa-miR-155-5p, finding a high prevalence of hosts immune response-related genes; moreover, several mRNAs involved in the viral infective process were also identified as targets for this miRNA. Our results suggest a relevant role for miR-155-5p in Salmo salar during an ISAV infection as a regulator of the immune response to the virus.

Original languageEnglish
Article number104109
JournalDevelopmental and Comparative Immunology
Volume122
DOIs
StatePublished - Sep 2021
Externally publishedYes

Keywords

  • Immune response
  • ISAV
  • miRNA
  • Salmo salar

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