Hepcidin, Cathelicidin-1 and IL-8 as immunological markers of responsiveness in early developmental stages of rainbow trout

Paula A. Santana, Fanny Guzmán, Juan C. Forero, Omar F. Luna, LUIS ALBERTO MERCADO VIANCO

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

During the early developmental stage of salmonids, high mortality occurs largely as a result of pathogens. These cause low immune competence in fry, producing disease, decreasing production and finally leading to economic losses. Therefore, the aim of this study was to characterise the developmental stages in which rainbow trout acquires immune response capability when challenged with LPS from Pseudomona aeruginosa for 8 h, studying the hepcidin, cathelicidin-1 and IL-8. Total RNA was extracted from fry at 34, 42, 56 and 66 days post hatching (dph). Hepcidin and cathelicidin-1 transcripts were detected only at days 34 and 42, whereas the IL-8 transcript was detected from day 34 to day 66. To analyse the protein expression in the fry, polyclonal anti-peptide antibodies were generated in rabbit. These three immune sera demonstrated the ability to recognise the whole molecule in biological samples. Immunofluorescence showed that skin, gills and intestine mainly responded to the LPS challenge, indicating that these portals of pathogen entry are capturing LPS. This study constitutes a valuable approach, since it has the potential to identify molecules with biological activity that can be used to evaluate the status of fry in culture.

Original languageEnglish
Pages (from-to)48-57
Number of pages10
JournalDevelopmental and Comparative Immunology
Volume62
DOIs
StatePublished - 1 Sep 2016

Keywords

  • Antimicrobial peptides
  • Fish innate immunity
  • LPS-challenge
  • Trout fry

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