HIF1α-dependent metabolic reprogramming governs mesenchymal stem/stromal cell immunoregulatory functions

Rafael Contreras-Lopez, Roberto Elizondo-Vega, Maria Jose Paredes, Noymar Luque-Campos, Maria Jose Torres, Gautier Tejedor, Ana Maria Vega-Letter, Aliosha Figueroa-Valdés, Carolina Pradenas, Karina Oyarce, Christian Jorgensen, Maroun Khoury, Maria de los Angeles Garcia-Robles, Claudia Altamirano, Farida Djouad, Patricia Luz-Crawford

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35 Scopus citations


Hypoxia-inducible factor 1 α (HIF1α), a regulator of metabolic change, is required for the survival and differentiation potential of mesenchymal stem/stromal cells (MSC). Its role in MSC immunoregulatory activity, however, has not been completely elucidated. In the present study, we evaluate the role of HIF1α on MSC immunosuppressive potential. We show that HIF1α silencing in MSC decreases their inhibitory potential on Th1 and Th17 cell generation and limits their capacity to generate regulatory T cells. This reduced immunosuppressive potential of MSC is associated with a metabolic switch from glycolysis to OXPHOS and a reduced capacity to express or produce some immunosuppressive mediators including Intercellular Adhesion Molecule (ICAM), IL-6, and nitric oxide (NO). Moreover, using the Delayed-Type Hypersensitivity murine model (DTH), we confirm, in vivo, the critical role of HIF1α on MSC immunosuppressive effect. Indeed, we show that HIF1α silencing impairs MSC capacity to reduce inflammation and inhibit the generation of pro-inflammatory T cells. This study reveals the pivotal role of HIF1α on MSC immunosuppressive activity through the regulation of their metabolic status and identifies HIF1α as a novel mediator of MSC immunotherapeutic potential.

Original languageEnglish
Pages (from-to)8250-8264
Number of pages15
JournalFASEB Journal
Issue number6
StatePublished - 1 Jun 2020


  • HIF1α
  • MSCs
  • glycolytic
  • immunomodulation
  • immunosuppression
  • metabolic reprogramming
  • metabolism


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