High glucose and low specific cell growth but not mild hypothermia improve specific r-protein productivity in chemostat culture of CHO cells

Agriculture & Biology

• biopharmaceuticals 9%
• cell cycle 7%
• cell growth 53%
• cell lines 5%
• cells 20%
• Chinese hamsters 68%
• glucose 38%
• hypothermia 75%
• lactates 7%
• metabolism 9%
• mitosis 7%
• multivariate analysis 8%
• post-translational modification 8%
• protein folding 9%
• proteins 2%
• recombinant proteins 62%
• specific growth rate 31%
• t-plasminogen activator 35%
• temperature 9%
• therapeutics 6%

Medicine & Life Sciences

• Biological Products 7%
• Cell Count 6%
• Cell Culture Techniques 6%
• Cell Cycle 6%
• Cell Division 7%
• Cell Line 4%
• Cricetulus 68%
• G2 Phase 8%
• Glucose 40%
• Growth 32%
• Hypothermia 67%
• Lactic Acid 7%
• Multivariate Analysis 5%
• Ovary 56%
• Post Translational Protein Processing 8%
• Protein Folding 9%
• Proteins 2%
• Recombinant Proteins 63%
• Temperature 17%
• Therapeutics 1%
• Tissue Plasminogen Activator 25%

Chemistry

• Chemostats 100%
• Dilution 19%
• Glucose 47%
• Lactate 8%
• Modification 4%
• Pharmacological Metabolism 12%
• Protein 26%
• Protein Folding 8%
• Reaction Yield 3%