Identification and isolation of infective filamentous particles in Infectious Salmon Anemia Virus (ISAV)

Ramón Ramírez, Sergio H. Marshall

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

The infectious salmon anemia virus (ISAV) is an aquatic pathogen that is a member of the Orthomyxoviridae family with lethal hemorrhagic potential. Although it affects other species of salmonid fish, ISAV only causes disease in Atlantic salmon (Salmo salar) specimens in sea water. In spite of the fact that the virus has been described as enveloped with icosahedral symmetry, viral like particles with anomalous morphology have been observed in field samples, this we have not been able to recover then in adequate quantities for full demonstration. We report a procedure to concentrate and recover these novel forms of the virus, comparing two cell lines from different origins, demonstrating that these forms were preferentially expressed in cells of epithelial origin.

Original languageEnglish
Pages (from-to)219-224
Number of pages6
JournalMicrobial Pathogenesis
Volume117
DOIs
StatePublished - Apr 2018
Externally publishedYes

Keywords

  • Epithelial cells
  • Filamentous particles
  • ISAV
  • Morphology
  • Salmo salar

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