IFIT5 participates in the antiviral mechanisms of rainbow trout red blood cells

Veronica Chico, Maria Elizabhet Salvador-Mira, Ivan Nombela, Sara Puente-Marin, Sergio Ciordia, María Carmen Mena, Luis Perez, Julio Coll, Fanny Guzman, Jose Antonio Encinar, LUIS ALBERTO MERCADO VIANCO, Maria del Mar Ortega-Villaizan

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Viral hemorrhagic septicemia virus (VHSV) infection appears to be halted in rainbow trout nucleated red blood cells (RBCs). Diverse mechanisms are thought to be related to the antiviral immune response of rainbow trout RBCs to VHSV. However, the specific rainbow trout RBC proteins that interact directly with VHSV are still unknown. In an attempt to identify VHSV-RBC protein interactions, we characterized the immunoprecipitated (IP) proteome of RBCs exposed to VHSV using an antibody against the N protein of VHSV. The IP proteomic characterization identified 31 proteins by mass spectrometry analysis. Among them, we identified interferon-induced protein with tetratricopeptide repeats 5 (IFIT5), a protein belonging to a family of proteins that are induced after the production of type I interferon. Importantly, IFIT5 has been implicated in the antiviral immune response. We confirmed the participation of IFIT5 in the rainbow trout RBC antiviral response by examining the expression profile of IFIT5 in RBCs after VHSV exposure at transcriptional and protein levels. We detected a correlation between the highest IFIT5 expression levels and the decline in VHSV replication at 6 h post-exposure. In addition, silencing ifit5 resulted in a significant increase in VHSV replication in RBCs. Moreover, an increase in VHSV replication was observed in RBCs when the IFIT5 RNA-binding pocket cavity was modulated by using a natural compound from the SuperNatural II database. We performed a proximity ligation assay and detected a significant increase in positive cells among VHSV-exposed RBCs compared to unexposed RBCs, indicating protein-protein colocalization between IFIT5 and the glycoprotein G of VHSV. In summary, these results suggest a possible role of IFIT5 in the antiviral response of RBCs against VHSV.

Original languageEnglish
Article number613
JournalFrontiers in Immunology
Volume10
Issue numberMAR
DOIs
StatePublished - 1 Jan 2019

Keywords

  • Antiviral immune response
  • Erythrocyte
  • IFIT5
  • Immunoprecipitate
  • Proteomic
  • Rainbow trout
  • Red blood cells
  • VHSV

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