TY - JOUR
T1 - Immune and proteomic responses to the soybean meal diet in skin and intestine mucus of Atlantic salmon (Salmo salar L.)
AU - Djordjevic, Brankica
AU - Morales-Lange, Byron
AU - Øverland, Margareth
AU - MERCADO VIANCO, LUIS ALBERTO
AU - Lagos, Leidy
N1 - Funding Information:
This study was funded by Foods of Norway, a Centre for Research‐based Innovation (237841/030), GutIntraPath (RCN 294527), Trained immunity and nutritional programming for resilient salmon (RCN 294821) and Postdoctoral program from the National Research and Development Agency of Chile (ANID‐Chile 74200139).
Publisher Copyright:
© 2021 The Authors. Aquaculture Nutrition published by John Wiley & Sons Ltd.
Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2021
Y1 - 2021
N2 - The main objective of this study was to increase the knowledge about the mucosal immunity of Salmo salar, using soybean meal-induced enteritis as a model of inflammation. A control fish meal (FM) and a diet containing 20% soybean meal (SBM) were fed to salmon for seven weeks in seawater. There was no growth difference between groups. However, histology of distal intestine (DI) showed a mild inflammation in the fish fed SBM. Proteomic results revealed differences between the diets. Among the proteins detected uniquely in DI mucus of SBM group, complement C5, Galectin and Glutathione synthetase are involved in innate and adaptive immunity, inflammation, redox signalling and detoxification of xenobiotics in mammals, and similar roles are hypothesized in salmon. Adenylosuccinate synthetase and putative aminopeptidase were uniquely detected in the skin mucus of SBM group. Trypsin enzymatic activity was significantly decreased in the DI of SBM group. Significantly higher production of immunoglobulin M and Mucin-like protein in DI mucus in SBM group was observed, while an increase in immunoglobulin D and lysozyme but decrease in chymotrypsin was detected in the skin mucus of the same group. We propose mucosal immunoglobulins as diagnostic biomarkers for assessment of novel feed ingredients and aquafeeds.
AB - The main objective of this study was to increase the knowledge about the mucosal immunity of Salmo salar, using soybean meal-induced enteritis as a model of inflammation. A control fish meal (FM) and a diet containing 20% soybean meal (SBM) were fed to salmon for seven weeks in seawater. There was no growth difference between groups. However, histology of distal intestine (DI) showed a mild inflammation in the fish fed SBM. Proteomic results revealed differences between the diets. Among the proteins detected uniquely in DI mucus of SBM group, complement C5, Galectin and Glutathione synthetase are involved in innate and adaptive immunity, inflammation, redox signalling and detoxification of xenobiotics in mammals, and similar roles are hypothesized in salmon. Adenylosuccinate synthetase and putative aminopeptidase were uniquely detected in the skin mucus of SBM group. Trypsin enzymatic activity was significantly decreased in the DI of SBM group. Significantly higher production of immunoglobulin M and Mucin-like protein in DI mucus in SBM group was observed, while an increase in immunoglobulin D and lysozyme but decrease in chymotrypsin was detected in the skin mucus of the same group. We propose mucosal immunoglobulins as diagnostic biomarkers for assessment of novel feed ingredients and aquafeeds.
KW - ELISA
KW - enzymatic activity
KW - histology
KW - proteomics
KW - Salmo salar
KW - SBMIE
UR - http://www.scopus.com/inward/record.url?scp=85099712263&partnerID=8YFLogxK
U2 - 10.1111/anu.13248
DO - 10.1111/anu.13248
M3 - Review article
AN - SCOPUS:85099712263
JO - Aquaculture Nutrition
JF - Aquaculture Nutrition
SN - 1353-5773
ER -