Implementation of an analytical method for the determination of inorganic arsenic species in occupationally exposed human urine samples and its toxic effects on epithelial cells of renal collecting tubule

Nicole Roldán, Nicolás Salinas-Parra, ALEXIS ANTONIO GONZALEZ PARRA, Flavia Cifuentes-Araneda, Helen Arias, Manuel Bravo, Waldo Quiroz

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

In this study a method was implemented for the determination of total As and its inorganic species in human urine by HPLC-HG-AFS. The method was applied to workers occupationally exposed to mining and thermoelectric operations. Results shows high levels of As in urine up to 188±4 μg L-1 and As(III) up to 10.7±0.6 μg L-1. Toxic effects of As(III) at the concentration levels founded in human urine were evaluated over the morphology and fibrosis of renal epithelial collecting duct cells in vitro. Our results suggest that urinary tract epithelial renal tubular cells exposed to this concentration of As(III) result in epithelial-mesenchymal transition.

Original languageEnglish
Pages (from-to)3214-3218
Number of pages5
JournalJournal of the Chilean Chemical Society
Volume61
Issue number4
DOIs
StatePublished - 1 Jan 2016

Keywords

  • Arsenic
  • Renal cells
  • Speciation
  • Toxic effects
  • Urine

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