Implementation of an analytical method for the determination of inorganic arsenic species in occupationally exposed human urine samples and its toxic effects on epithelial cells of renal collecting tubule

Nicole Roldán; Nicolás Salinas-Parra; Alexis A. Gonzalez; Flavia Cifuentes-Araneda; Helen Arias; Manuel Bravo; Waldo Quiroz

doi:10.4067/S0717-97072016000400013

Implementation of an analytical method for the determination of inorganic arsenic species in occupationally exposed human urine samples and its toxic effects on epithelial cells of renal collecting tubule

Nicole Roldán, Nicolás Salinas-Parra, Alexis A. Gonzalez, Flavia Cifuentes-Araneda, Helen Arias, Manuel Bravo, Waldo Quiroz

INSTITUTE OF CHEMISTRY

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Abstract

In this study a method was implemented for the determination of total As and its inorganic species in human urine by HPLC-HG-AFS. The method was applied to workers occupationally exposed to mining and thermoelectric operations. Results shows high levels of As in urine up to 188±4 μg L^-1 and As(III) up to 10.7±0.6 μg L^-1. Toxic effects of As(III) at the concentration levels founded in human urine were evaluated over the morphology and fibrosis of renal epithelial collecting duct cells in vitro. Our results suggest that urinary tract epithelial renal tubular cells exposed to this concentration of As(III) result in epithelial-mesenchymal transition.

Original language	English
Pages (from-to)	3214-3218
Number of pages	5
Journal	Journal of the Chilean Chemical Society
Volume	61
Issue number	4
DOIs	https://doi.org/10.4067/S0717-97072016000400013
State	Published - 2016

Keywords

Arsenic
Renal cells
Speciation
Toxic effects
Urine

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Roldán, N., Salinas-Parra, N., Gonzalez, A. A., Cifuentes-Araneda, F., Arias, H., Bravo, M., & Quiroz, W. (2016). Implementation of an analytical method for the determination of inorganic arsenic species in occupationally exposed human urine samples and its toxic effects on epithelial cells of renal collecting tubule. Journal of the Chilean Chemical Society, 61(4), 3214-3218. https://doi.org/10.4067/S0717-97072016000400013

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title = "Implementation of an analytical method for the determination of inorganic arsenic species in occupationally exposed human urine samples and its toxic effects on epithelial cells of renal collecting tubule",

abstract = "In this study a method was implemented for the determination of total As and its inorganic species in human urine by HPLC-HG-AFS. The method was applied to workers occupationally exposed to mining and thermoelectric operations. Results shows high levels of As in urine up to 188±4 μg L-1 and As(III) up to 10.7±0.6 μg L-1. Toxic effects of As(III) at the concentration levels founded in human urine were evaluated over the morphology and fibrosis of renal epithelial collecting duct cells in vitro. Our results suggest that urinary tract epithelial renal tubular cells exposed to this concentration of As(III) result in epithelial-mesenchymal transition.",

keywords = "Arsenic, Renal cells, Speciation, Toxic effects, Urine",

author = "Nicole Rold{\'a}n and Nicol{\'a}s Salinas-Parra and Gonzalez, {Alexis A.} and Flavia Cifuentes-Araneda and Helen Arias and Manuel Bravo and Waldo Quiroz",

year = "2016",

doi = "10.4067/S0717-97072016000400013",

language = "English",

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Roldán, N, Salinas-Parra, N, Gonzalez, AA, Cifuentes-Araneda, F, Arias, H, Bravo, M & Quiroz, W 2016, 'Implementation of an analytical method for the determination of inorganic arsenic species in occupationally exposed human urine samples and its toxic effects on epithelial cells of renal collecting tubule', Journal of the Chilean Chemical Society, vol. 61, no. 4, pp. 3214-3218. https://doi.org/10.4067/S0717-97072016000400013

Implementation of an analytical method for the determination of inorganic arsenic species in occupationally exposed human urine samples and its toxic effects on epithelial cells of renal collecting tubule. / Roldán, Nicole; Salinas-Parra, Nicolás; Gonzalez, Alexis A. et al.
In: Journal of the Chilean Chemical Society, Vol. 61, No. 4, 2016, p. 3214-3218.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Implementation of an analytical method for the determination of inorganic arsenic species in occupationally exposed human urine samples and its toxic effects on epithelial cells of renal collecting tubule

AU - Roldán, Nicole

AU - Salinas-Parra, Nicolás

AU - Gonzalez, Alexis A.

AU - Cifuentes-Araneda, Flavia

AU - Arias, Helen

AU - Bravo, Manuel

AU - Quiroz, Waldo

PY - 2016

Y1 - 2016

N2 - In this study a method was implemented for the determination of total As and its inorganic species in human urine by HPLC-HG-AFS. The method was applied to workers occupationally exposed to mining and thermoelectric operations. Results shows high levels of As in urine up to 188±4 μg L-1 and As(III) up to 10.7±0.6 μg L-1. Toxic effects of As(III) at the concentration levels founded in human urine were evaluated over the morphology and fibrosis of renal epithelial collecting duct cells in vitro. Our results suggest that urinary tract epithelial renal tubular cells exposed to this concentration of As(III) result in epithelial-mesenchymal transition.

AB - In this study a method was implemented for the determination of total As and its inorganic species in human urine by HPLC-HG-AFS. The method was applied to workers occupationally exposed to mining and thermoelectric operations. Results shows high levels of As in urine up to 188±4 μg L-1 and As(III) up to 10.7±0.6 μg L-1. Toxic effects of As(III) at the concentration levels founded in human urine were evaluated over the morphology and fibrosis of renal epithelial collecting duct cells in vitro. Our results suggest that urinary tract epithelial renal tubular cells exposed to this concentration of As(III) result in epithelial-mesenchymal transition.

KW - Arsenic

KW - Renal cells

KW - Speciation

KW - Toxic effects

KW - Urine

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JF - Journal of the Chilean Chemical Society

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ER -

Roldán N, Salinas-Parra N, Gonzalez AA, Cifuentes-Araneda F, Arias H, Bravo M et al. Implementation of an analytical method for the determination of inorganic arsenic species in occupationally exposed human urine samples and its toxic effects on epithelial cells of renal collecting tubule. Journal of the Chilean Chemical Society. 2016;61(4):3214-3218. doi: 10.4067/S0717-97072016000400013

Implementation of an analytical method for the determination of inorganic arsenic species in occupationally exposed human urine samples and its toxic effects on epithelial cells of renal collecting tubule

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Keywords

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