Improvement of Efficiency in the Enzymatic Synthesis of Lactulose Palmitate

Claudia Bernal, Andres Illanes, Lorena Wilson

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35 Scopus citations


Sugar esters are considered as surfactants due to its amphiphilic balance that can lower the surface tension in oil/water mixtures. Enzymatic syntheses of these compounds are interesting both from economic and environmental considerations. A study was carried out to evaluate the effect of four solvents, temperature, substrate molar ratio, biocatalyst source, and immobilization methodology on the yield and specific productivity of lactulose palmitate monoester synthesis. Lipases from Pseudomonas stutzeri (PsL) and Alcaligenes sp. (AsL), immobilized in porous silica functionalized with octyl groups (adsorption immobilization, OS) and with glyoxyl-octyl groups (both adsorption and covalent immobilization, OGS), were used. The highest lactulose palmitate yields were obtained at 47 C in acetone, for all biocatalysts, while the best lactulose:palmitic acid molar ratio differed according to the immobilization methodology, being 1:1 for AsL-OGS biocatalyst (20.7 ± 3%) and 1:3 for the others (30-50%).

Original languageEnglish
Pages (from-to)3716-3724
Number of pages9
JournalJournal of Agricultural and Food Chemistry
Issue number14
StatePublished - 15 Apr 2015


  • Immobilization
  • Lactulose
  • Lipase
  • Silica support
  • Sugar fatty acid ester


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