In vitro propagation of easter island curcuma longa from rhizome explants using temporary immersion system

María José Marchant, Paula Molina, Miriam Montecinos, Leda Guzmán, Cristobal Balada, Claudia Fassio, Mónica Castro

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Curcuma longa (C. longa) is widely known for its medicinal properties. However, the potential overexploitation of this plant raises doubts about its long‐term survival on Rapa Nui. Micro-propagation using a temporary immersion system (TIS) could be the basis for developing a cost-effective and highly productive method of large‐scale cultivation of this plant. Our objective was to develop and refine the in vitro multiplication system for mass propagation of C. longa, and thus help restore the fragile ecosystem of Rapa Nui. Three parameters were evaluated: number of ex-plants per flask, flask capacity, and LEDs spectrum. For each parameter evaluated, four aspects were analyzed: fresh weight per plant, number of shoots, percentage of non‐sprouting explants, and the proliferation rate. The use of 30 explants per two‐liter flask results in more plants with high fresh biomass than other configurations. In addition, LEDs with a red:blue ratio of 2:1 provided the best lighting conditions for in vitro propagation and positively affected C. longa proliferation and rooting. Therefore, our results show that 30 explants per two‐liter flask and an LED source with a red:blue ratio of 2:1 allow a higher number of C. longa plants to be obtained using TIS.

Original languageEnglish
Article number2121
JournalAgronomy
Volume11
Issue number11
DOIs
StatePublished - Nov 2021
Externally publishedYes

Keywords

  • Automatization
  • Conservation
  • LED light
  • Micropropagation
  • Rapa nui
  • Turmeric

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