Indoleamine 2,3-dioxygenase: First evidence of expression in rainbow trout (Oncorhynchus mykiss)

Jimena Cortés, Claudio Alvarez, Paula Santana, Elisa Torres, LUIS ALBERTO MERCADO VIANCO

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

The role of enzymes as active antimicrobial agents of the innate immunity in teleost fish is proposed in diverse works. Secretion of Indoleamine 2,3-dioxygenase (IDO) has been described in higher vertebrates; it degrades L-tryptophan in extracellular environments associated mainly with mucosal organs. The effect of IDO on decreasing amino acid concentration may inhibit the growth of potential pathogens. In fish the study of this molecule is still. Here we report the identification of an Onchorhyncus mykiss IDO homologue (OmIDO). IDO was cloned, sequenced, and the primary structure shows conservation of key functional sites. The constitutive expression is altered when the fish is challenged with LPS as a pathogen-associated molecular pattern (PAMPs). Up-regulation of IDO was shown preferentially in the fish's mucosal cells. In order to obtain evidence of a possible regulation mechanism, an in vitro cell model was used for to show that OmIDO is induced by rIFN. These study has identified a Indoleamine 2,3-dyoxigenase in O. mykiss will contribute to expands our knowledge of the function this protein in fish immune response. These findings allow to propose the use of OmIDO as a molecular indicator of strength of the animal's immune response and wellbeing.

Original languageEnglish
Pages (from-to)73-78
Number of pages6
JournalDevelopmental and Comparative Immunology
Volume65
DOIs
StatePublished - 1 Dec 2016

Keywords

  • Fish IFN-γ
  • Fish innate immunity
  • Immune regulation
  • ITIMs motifs
  • Tryptophan metabolism

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