TY - JOUR
T1 - Inherent and stress-induced responses of fine root morphology and anatomy in commercial grapevine rootstocks with contrasting drought resistance
AU - Reingwirtz, Idan
AU - Uretsky, Jake
AU - CUNEO ARRATIA, ITALO FABRIZZIO
AU - Knipfer, Thorsten
AU - Reyes, Clarissa
AU - Walker, M. Andrew
AU - McElrone, Andrew J.
N1 - Funding Information:
Funding: Advance Light Source (ALS) is supported by the Director, Office of Science, Office of Basic Energy Science, of the US Department of Energy under contract no. DE-AC02-05CH11231. I.F.C. gratefully acknowledges the Fondecyt-ANID grant N◦11180102. Also, this work was supported by USDA-ARS CRIS project 2032-21220-006-00D.
Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/6
Y1 - 2021/6
N2 - Some grapevine rootstocks perform better than others during and after drought events, yet it is not clear how inherent and stress-induced differences in root morphology and anatomy along the length of fine roots are involved in these responses. Using a variety of growing conditions and plant materials, we observed significant differences in root diameter, specific root length (SRL) and root diameter distribution between two commonly used commercial grapevine rootstocks: Richter 110 (110R; drought resistant) and Millardet et de Grasset 101-14 (101-14Mgt; drought sensitive). The 110R consistently showed greater root diameters with smaller SRL and proportion of root length comprised of fine lateral roots. The 110R also exhibited significantly greater distance from tip to nearest lateral, longer white root length, and larger proportion of root length that is white under drought stress. Mapping of fine root cortical lacunae showed similar patterns between the rootstocks; mechanical failure of cortical cells was common in the maturation zone, limited near the root tip, and increased with drought stress for both genotypes; however, lacuna formed under wetter soil conditions in 110R. Results suggest that drought resistance in grapevine rootstocks is associated with thick, limitedly branched roots with a larger proportion of white-functional roots that tend to form lacuna under more mild water deficit, all of which likely favor continued resource acquisition at depth.
AB - Some grapevine rootstocks perform better than others during and after drought events, yet it is not clear how inherent and stress-induced differences in root morphology and anatomy along the length of fine roots are involved in these responses. Using a variety of growing conditions and plant materials, we observed significant differences in root diameter, specific root length (SRL) and root diameter distribution between two commonly used commercial grapevine rootstocks: Richter 110 (110R; drought resistant) and Millardet et de Grasset 101-14 (101-14Mgt; drought sensitive). The 110R consistently showed greater root diameters with smaller SRL and proportion of root length comprised of fine lateral roots. The 110R also exhibited significantly greater distance from tip to nearest lateral, longer white root length, and larger proportion of root length that is white under drought stress. Mapping of fine root cortical lacunae showed similar patterns between the rootstocks; mechanical failure of cortical cells was common in the maturation zone, limited near the root tip, and increased with drought stress for both genotypes; however, lacuna formed under wetter soil conditions in 110R. Results suggest that drought resistance in grapevine rootstocks is associated with thick, limitedly branched roots with a larger proportion of white-functional roots that tend to form lacuna under more mild water deficit, all of which likely favor continued resource acquisition at depth.
KW - Fine root diameter
KW - Fine roots
KW - Root populations cortical lacunae
KW - Specific root length
UR - http://www.scopus.com/inward/record.url?scp=85106922076&partnerID=8YFLogxK
U2 - 10.3390/plants10061121
DO - 10.3390/plants10061121
M3 - Article
AN - SCOPUS:85106922076
VL - 10
JO - Plants
JF - Plants
SN - 2223-7747
IS - 6
M1 - 1121
ER -