Integrated transcriptomic and proteomic analysis of red blood cells from rainbow trout challenged with VHSV point towards novel immunomodulant targets

Ivan Nombela, Marina Lopez-Lorigados, Maria Elizabeth Salvador-Mira, Sara Puente-Marin, Veronica Chico, Sergio Ciordia, Maria Carmen Mena, Luis Mercado, Julio Coll, Luis Perez, Maria del Mar Ortega-Villaizan

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Teleost red blood cells (RBCs) are nucleated and therefore can propagate cellular responses to exogenous stimuli. RBCs can mount an immune response against a variety of fish viruses, including the viral septicemia hemorrhagic virus (VHSV), which is one of the most prevalent fish viruses resulting in aquaculture losses. In this work, RBCs from blood and head kidney samples of rainbow trout challenged with VHSV were analyzed via transcriptomic and proteomic analyses. We detected an overrepresentation of differentially expressed genes (DEGs) related to the type I interferon response and signaling in RBCs from the head kidney and related to complement activation in RBCs from blood. Antigen processing and presentation of peptide antigen was overrepresented in RBCs from both tissues. DEGs shared by both tissues showed an opposite expression profile. In summary, this work has demonstrated that teleost RBCs can modulate the immune response during an in vivo viral infection, thus implicating RBCs as cell targets for the development of novel immunomodulants.

Original languageEnglish
Article number63
JournalVaccines
Volume7
Issue number3
DOIs
StatePublished - Sep 2019
Externally publishedYes

Keywords

  • Antigen presentation
  • Complement
  • IFIT5
  • Interferon
  • Mx
  • Proteome
  • Red blood cells
  • Transcriptome
  • VHSV; rhabdoviruses
  • erythrocytes
  • β-defensin 1

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