Interactive 360° video to “Polyphenolic extracts of walnut (Juglans regia) green husk containing juglone inhibit the growth of HL-60 cells and induce apoptosis”

Carmen Soto-Maldonado, Mauricio Vergara-Castro, John Jara-Quezada, Eduardo Caballero-Valdés, Andrea Müller-Pavez, María Elvira Zúñiga-Hansen, Claudia Altamirano

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Juglone is a naphthoquinone currently obtained by chemical synthesis with biological activities including antitumor activity. Additionally, juglone is present in the green husk of walnut, which suggests evaluating the effect of GH extracts on carcinogenic cell lines. Results: Walnut green husk ethanolic extract was obtained as 169.1 mg juglone/100 g Green Husk and antioxidant activity (ORAC) of 44,920 μmol Trolox Equivalent/100 g DW Green Husk. At 1 μM juglone in HL-60 cell culture, green husk extract showed an antiproliferative effect, but pure juglone did not; under these conditions, normal fibroblast cells were not affected. A dose-dependent effect on mitochondrial membrane potential loss was observed. Apoptosis of HL-60 was detected at 10 μM juglone. Despite high ORAC values, neither purified juglone nor the extract showed protective effects on HL-60 cells under oxidative conditions. Conclusions: Green husk extract generates an antiproliferative effect in HL-60 cells, which is related to an induction of the early stages of apoptosis and a loss of mitochondrial membrane potential. The normal cells were not affected when juglone is present at concentrations of 1 μM, while at higher concentrations, there is loss of viability of both cancerous and healthy cells. How to cite: Soto-Maldonado C, Vergara-Castro M, Jara-Quezada J, et al. Interactive 360° video to: Polyphenolic extracts of walnut (Juglans regia) green husk containing juglone inhibit the growth of HL-60 cells and induce apoptosis. Electron J Biotechnol 2022;57. https://doi.org/10.1016/j.ejbt.2022.04.002.

Original languageEnglish
Pages (from-to)52-53
Number of pages2
JournalElectronic Journal of Biotechnology
Volume57
DOIs
StatePublished - May 2022
Externally publishedYes

Keywords

  • 360°
  • 360° video
  • Antiproliferative
  • Apoptosis
  • Green husk
  • HL-60 cells
  • Immersive
  • Interactive
  • Juglans regia
  • Juglone
  • Walnut

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